Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

QTL Mapping of Soybean (Glycine max) Vine Growth Habit Trait

Jian Song
*,† ORCID logo ,
Xuezhen Yang
† ,
Kanglin Liu
† ,
Yijie Chen
,
Yajun Xiong
,
Qichao Yang
,
Jing Wang
,
Zhihao Zhang
,
Caiyu Wu
,
Jun Wang
* ,
Lijuan Qiu
*
†
These authors have contributed equally to this work.
Version 1 : Received: 30 August 2023 / Approved: 30 August 2023 / Online: 31 August 2023 (10:39:34 CEST)

A peer-reviewed article of this Preprint also exists.

Song, J.; Liu, K.; Yang, X.; Chen, Y.; Xiong, Y.; Yang, Q.; Wang, J.; Zhang, Z.; Wu, C.; Wang, J.; Qiu, L. QTL Mapping of Soybean (Glycine max) Vine Growth Habit Trait. Int. J. Mol. Sci. 2023, 24, 14770. Song, J.; Liu, K.; Yang, X.; Chen, Y.; Xiong, Y.; Yang, Q.; Wang, J.; Zhang, Z.; Wu, C.; Wang, J.; Qiu, L. QTL Mapping of Soybean (Glycine max) Vine Growth Habit Trait. Int. J. Mol. Sci. 2023, 24, 14770.

Abstract

Vine growth habit (VGH) is a notable property of wild soybean plants that also holds a high degree of importance in the context of domestication as it can preclude the use of these wild cultivars for the breeding and improvement of domesticated soybean. Here, a bulked segregant analysis (BSA) approach was employed to study the genetic etiology of VGH in soybean plants by integrating linkage mapping and population sequencing approaches. To develop a recombinant inbred line (RIL) population, the cultivated Zhongdou41 (ZD41) soybean cultivar was bred with ZYD02787, a wild soybean accession. The VGH status of each line in the resultant population was assessed, ultimately leading to the identification of 6 and 9 QTLs from the BSA sequencing of the F4 population and F6- F8 population sequence mapping, respectively. One QTL shared across these analyzed generations was detected on chromosome 19. Three other QTLs detected by BSA-seq were validated and localized to 90.93 kb, 2.9 Mb, and 602.08 kb regions of chromosomes 6 and 13, respectively harboring 14, 53, and 4 genes. Four consistent VGH-related QTLs located on chromosomes 10, 13, and 19 were detected by both analytical approaches in a minimum of two environments, while an additional five loci on chromosomes 2, 10, and 18 were detected in at least two environments only via ICIM mapping. Of the detected loci, five had been reported previously whereas six represent novel QTLs. Together, these data offer new insight into the genetic basis for VGH in soybean plates, providing a rational basis to inform the use of wild accessions in future breeding efforts.

Keywords

Glycine soja; Vine growth habit; QTL mapping

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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