Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

QTL and Candidate Genes that Control Seed Sugars Contents in the Soybean ‘Forrest’ By ‘Williams 82’ RIL Population

Dounya Knizia
ORCID logo ,
Nacer Bellaloui
,
Jiazheng Yuan
,
Naoufal Lakhssassi
ORCID logo ,
Erdem Anil
,
Tri Vuong
,
Mohamed G. Embaby
ORCID logo ,
Henry T Nguyen
ORCID logo ,
Alemu Mengistu
,
Khalid Meksem
ORCID logo ,
Abdelmajid My Kassem
* ORCID logo
Version 1 : Received: 29 August 2023 / Approved: 30 August 2023 / Online: 31 August 2023 (03:50:41 CEST)

A peer-reviewed article of this Preprint also exists.

Knizia, D.; Bellaloui, N.; Yuan, J.; Lakhssasi, N.; Anil, E.; Vuong, T.; Embaby, M.; Nguyen, H.T.; Mengistu, A.; Meksem, K.; Kassem, M.A. Quantitative Trait Loci and Candidate Genes That Control Seed Sugars Contents in the Soybean ‘Forrest’ by ‘Williams 82’ Recombinant Inbred Line Population. Plants 2023, 12, 3498. Knizia, D.; Bellaloui, N.; Yuan, J.; Lakhssasi, N.; Anil, E.; Vuong, T.; Embaby, M.; Nguyen, H.T.; Mengistu, A.; Meksem, K.; Kassem, M.A. Quantitative Trait Loci and Candidate Genes That Control Seed Sugars Contents in the Soybean ‘Forrest’ by ‘Williams 82’ Recombinant Inbred Line Population. Plants 2023, 12, 3498.

Abstract

Soybean seed sugars are among the most abundant beneficial compounds for human and animal consumption in soybean seeds. Higher seed sugars such as sucrose are desirable as it contributes to taste and flavor in soy-based food. Therefore, the objectives of this study were to use ‘Forrest’ by ‘Williams 82’ (F×W82) recombinant inbred line (RIL) soybean population (n=309) to identify quantitative trait loci (QTL) and candidate genes that control seed sugar (sucrose, stachyose, and raffinose) contents in two environments (North Carolina and Illinois) over two years (2018 and 2020). A total of 26 QTL that control seed sugars contents were identified and mapped on 16 soybean chromosomes (chrs.). Interestingly, five QTL regions were identified in both locations, Illinois and North Carolina, in this study on chrs. 2, 5, 13, 17, and 20. Amongst 57 candidate genes identified in this study, 16 were located within 10 Megabase (MB) of the identified QTL. Amongst them a cluster of four genes involved in the sugars’ pathway was collocated within 6 MB with two QTL that were detected in this study on chr. 17. Further functional validation of the identified genes could be beneficial in breeding programs to produce soybean lines with high beneficial sucrose and low raffinose family oligosaccharides.

Keywords

soybean; RIL; Forrest; Williams 82; linkage map; RFOs; sucrose; raffinose; stachyose; SNP

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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