Version 1
: Received: 29 August 2023 / Approved: 30 August 2023 / Online: 30 August 2023 (07:16:25 CEST)
How to cite:
Chen, T.-H.; Lin, A.-H.; Yeh, C.-T.; Wu, C.-H. Genotyping of Helicobacter pylori ureC Gene: Connection of Its Association with Gastric Cancer in Taiwan. Preprints2023, 2023082029. https://doi.org/10.20944/preprints202308.2029.v1
Chen, T.-H.; Lin, A.-H.; Yeh, C.-T.; Wu, C.-H. Genotyping of Helicobacter pylori ureC Gene: Connection of Its Association with Gastric Cancer in Taiwan. Preprints 2023, 2023082029. https://doi.org/10.20944/preprints202308.2029.v1
Chen, T.-H.; Lin, A.-H.; Yeh, C.-T.; Wu, C.-H. Genotyping of Helicobacter pylori ureC Gene: Connection of Its Association with Gastric Cancer in Taiwan. Preprints2023, 2023082029. https://doi.org/10.20944/preprints202308.2029.v1
APA Style
Chen, T. H., Lin, A. H., Yeh, C. T., & Wu, C. H. (2023). Genotyping of Helicobacter pylori ureC Gene: Connection of Its Association with Gastric Cancer in Taiwan. Preprints. https://doi.org/10.20944/preprints202308.2029.v1
Chicago/Turabian Style
Chen, T., Chau-Ting Yeh and Chi-Huan Wu. 2023 "Genotyping of Helicobacter pylori ureC Gene: Connection of Its Association with Gastric Cancer in Taiwan" Preprints. https://doi.org/10.20944/preprints202308.2029.v1
Abstract
Helicobacter pylori (H. pylori) is known to play a significant role in the development of peptic ulcer diseases and gastric cancer. Previous studies have reported the existence of polymorphism in the ureC gene of H. pylori. However, the relationship between genetic sequence variations in ureC and the pathogenesis of peptic ulcer diseases remains unclear. We aimed to investigate the associations between ureC sequence variations and the development of gastric ulcer and gastric cancer. PCR amplification and DNA sequencing to analyze ureC sequences, and conducted phylogenetic analysis using MEGA X software. Samples from Taiwanese patients with gastric ulcer and gastric cancer were included in this study. PCR was used to amplify the ureC gene from these samples, followed by DNA sequencing. The obtained sequences were then subjected to phylogenetic analysis using MEGA X software. In addition, ureC sequences from different geographical regions, including China and other countries, were included in the analysis for comparison. Analysis of the ureC se-quences from Taiwanese samples revealed that 6 out of 7 gastric ulcer samples clustered together in one group, while the sequences from 7 out of 8 gastric cancer samples were distributed across other groups. Phylogenetic analysis incorporating ureC sequences from different geographical regions showed that Taiwanese ureC sequences could be divided into two distinct groups, although the bootstrap values supporting this separation were low. Notably, the ureC sequences from China formed a distinct group with a high bootstrap value, separate from sequences from all other countries. There are two genotypes of ureC sequences present in Taiwanese samples, with one genotype showing a closer association with gastric cancer. Additionally, the ureC sequences from China appear to be unique and separate from those obtained from other countries, indicating the presence of genetic diversity in ureC sequences among H. pylori strains from different regions, potentially contributing to differences in disease outcomes. Further research is needed to elucidate the specific mechanisms by which ureC sequence variations may influence the pathogenesis of peptic ulcer diseases and gastric cancer.
Keywords
Helicobacter pylori; ureC; phylogenetic tree; gastric cancer
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
