Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Autophagy-Associated Markers LC3-II, ULK2 and microRNAs miR-21, miR-126 and miR-374 as a Potential Prognostic Indicator for Glioma Patients

Wajiha Amin
ORCID logo ,
Syed Ather Enam
,
Sufiyan Sufiyan
ORCID logo ,
Sana Naeem
,
Altaf Ali Laghari
,
Mohammad Hamza Bajwa
,
Kulsoom Ghias
,
Syed Hani Abidi
* ORCID logo ,
Nouman Mughal
*
Version 1 : Received: 25 August 2023 / Approved: 28 August 2023 / Online: 29 August 2023 (03:33:04 CEST)

How to cite: Amin, W.; Enam, S.A.; Sufiyan, S.; Naeem, S.; Laghari, A.A.; Bajwa, M.H.; Ghias, K.; Abidi, S.H.; Mughal, N. Autophagy-Associated Markers LC3-II, ULK2 and microRNAs miR-21, miR-126 and miR-374 as a Potential Prognostic Indicator for Glioma Patients. Preprints 2023, 2023081897. https://doi.org/10.20944/preprints202308.1897.v1 Amin, W.; Enam, S.A.; Sufiyan, S.; Naeem, S.; Laghari, A.A.; Bajwa, M.H.; Ghias, K.; Abidi, S.H.; Mughal, N. Autophagy-Associated Markers LC3-II, ULK2 and microRNAs miR-21, miR-126 and miR-374 as a Potential Prognostic Indicator for Glioma Patients. Preprints 2023, 2023081897. https://doi.org/10.20944/preprints202308.1897.v1

Abstract

Abstract: Autophagy has a dual role in gliomagenesis in a microRNA-modulated environment. We investigated the potential relevance of autophagy in glioma development and survival by exploring the association of autophagy-associated genes and microRNAs in low- and high-grade gliomas. Real-time PCR (qPCR) was used to determine the expression of genes and microRNAs in 50 fresh glioma tissues while Formalin-fixed paraf-fin-embedded tissues of the same patients were used for immunohistochemistry. The Mann-Whitney U-test test, Spearman correlation test, and Kaplan-Meier survival analysis were performed to evaluate the expression, association, and overall survival in patients respectively. The expression of LC3, AKT, and miR-21 was increased in high-grade glioma compared to low-grade glioma while ULK2 expression was decreased in high-grade glioma. A strong positive correlation was observed for ULK2 with UVRAG, PTEN, miR-7, and miR-100, while the moderate correlation with mTOR, Beclin1, miR-30, miR-204, miR-374, miR-21 and miR-126 in low-grade glioma, while a moderate positive correlation between ULK2 and PI3K, PTEN, ULK1, VPS34, mTOR, Beclin1, UVRAG, AKT and miR-374, and between AKT and ULK1, VPS34, UVRAG, and miR-7 in high-grade gliomas. The low ULK2 and LC3 expression group was significantly associated with better overall survival in gliomas while miR-21 overexpression showed a poor prognosis in glioma patients. Therefore, miR-21, ULK2, and LC3 may serve as prognostic biomarkers for survival outcomes in glioblastoma.

Keywords

Glioma; Prognostic markers; Autophagy; Micro-RNA; Overall Survival

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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