Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multi Drug Resistant and Heteroresistant Mycobacterium tuberculosis Isolates Variants from Patients in Rural Areas in Eastern Cape

Lindiwe M. Faye
* ORCID logo ,
Mojisola C. Hosu
,
Yanga Mbiza
,
Nomzi Lubuzo
,
Ncomeka Sineke
,
Thulani Knowledge Gumede
,
Carine Bokop
,
Bonnie Ifaedi
,
Ntandazo Dlatu
ORCID logo ,
Sandeep Vasaikar
ORCID logo ,
Teke Apalata
Version 1 : Received: 26 August 2023 / Approved: 28 August 2023 / Online: 29 August 2023 (03:33:51 CEST)

How to cite: Faye, L.M.; Hosu, M.C.; Mbiza, Y.; Lubuzo, N.; Sineke, N.; Gumede, T.K.; Bokop, C.; Ifaedi, B.; Dlatu, N.; Vasaikar, S.; Apalata, T. Multi Drug Resistant and Heteroresistant Mycobacterium tuberculosis Isolates Variants from Patients in Rural Areas in Eastern Cape. Preprints 2023, 2023081895. https://doi.org/10.20944/preprints202308.1895.v1 Faye, L.M.; Hosu, M.C.; Mbiza, Y.; Lubuzo, N.; Sineke, N.; Gumede, T.K.; Bokop, C.; Ifaedi, B.; Dlatu, N.; Vasaikar, S.; Apalata, T. Multi Drug Resistant and Heteroresistant Mycobacterium tuberculosis Isolates Variants from Patients in Rural Areas in Eastern Cape. Preprints 2023, 2023081895. https://doi.org/10.20944/preprints202308.1895.v1

Abstract

Multidrug-resistant tuberculosis emerged as a serious challenge to tuberculosis management and control. In the Eastern Cape, the Beijing variants are prevalent and a driving force of multidrug-resistant tuberculosis; hence, we investigated the distribution of gene mutations in Beijing strains compared to non-Beijing strains. Multidrug-resistant tuberculosis and heteroresistant isolates were identified in 412 sputum cultures by drug susceptibility testing. The isolates were analyzed for mutations in three genes associated with resistance to antituberculosis first-line drugs: katG and inhA promoters for isoniazid and rpoB for rifampicin. All isolates were genotyped by spoligotyping. There were more males than females and a more economically active age group in the study. The most prevalent mutations in rpoB resistance were in S531L, katG in S315Tb, and inhA in c-15tb. Heteroresistance was found in 18 isolates. Beijing variants were predominant. Most of the heteroresistant isolates were INH, with heteroresistance occurring more in the inhA gene mutation region c-15tb. Beijing and LAM variants were found more frequently in INH heteroresistant isolates. Mutations in katG S315Tb and rpoB S531L were higher in Beijing variants. The Beijing family is a major contributor to the epidemiological picture and accounts for most of the multidrug-resistant tuberculosis in the study area.

Keywords

keyword multidrug-resistant tuberculosis; gene mutations; heteroresistance; Beijing variants

Subject

Public Health and Healthcare, Primary Health Care

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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