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Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. Var. Capitata) Seedlings
Jia, K.; Zhan, Z.; Wang, B.; Wang, W.; Wei, W.; Li, D.; Huang, W.; Xu, Z. Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings. Horticulturae2023, 9, 1016.
Jia, K.; Zhan, Z.; Wang, B.; Wang, W.; Wei, W.; Li, D.; Huang, W.; Xu, Z. Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings. Horticulturae 2023, 9, 1016.
Jia, K.; Zhan, Z.; Wang, B.; Wang, W.; Wei, W.; Li, D.; Huang, W.; Xu, Z. Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings. Horticulturae2023, 9, 1016.
Jia, K.; Zhan, Z.; Wang, B.; Wang, W.; Wei, W.; Li, D.; Huang, W.; Xu, Z. Exogenous Selenium Enhances Cadmium Stress Tolerance by Improving Physiological Characteristics of Cabbage (Brassica oleracea L. var. capitata) Seedlings. Horticulturae 2023, 9, 1016.
Abstract
Cabbage (Brassica oleracea L. var. capitata) plays a very important role in the annual national vegetable supply and export trade. In recent years, the cadmium content of agricultural soils has increased. As a result, cabbage yields and quality have declined significantly. Studies have shown that selenium can counteract the harmful effects of cadmium on plants and can alleviate the stress caused by cadmium during the growth of plants. However, the mechanism of exogenous selenium application in the alleviation of cadmium stress in cabbage seedlings has not been thoroughly investigated. In this study, exogenous selenium (10μMol/L) was applied under cadmium (25μMol/L) stress and the physiological mechanisms such as biomass, photosynthetic pigment, leaf stomata parameters, selenium and cadmium content, chloroplast ultrastructure, active oxygen accumulation, leaf membrane esterification and antioxidant enzyme activity were determined. The protective mechanism of exogenous selenium on cabbage seedlings under cadmium stress was investigated. The re-sults showed that exogenous application of selenium could effectively alleviate the decrease in growth, photosynthetic pigment and gas exchange characteristics of cabbage seedlings under cadmium stress, im-prove cabbage root vitality, reduce root leaf cadmium content and alleviate cadmium stress-induced damage. Ultrastructural observation showed that cadmium stress caused the disruption of the internal structure of chloroplasts of cabbage leaves, while exogenous selenium treatment alleviated the chloroplast damage to some extent, improved the stability of the inner capsule membrane and alleviated the cadmium stress-induced damage to photosynthetic organs. Cadmium stress also caused oxidative damage and ex-cessive accumulation of ROS in the leaves of cabbage seedlings as evidenced by significant accumulation of O2-, H2O2, MDA and electrolyte leakage. On the other hand, after exogenous selenium treatment, cadmium stress- induced oxidative damage could be reduced by up-regulating the activities of antioxidant enzymes SOD, POD and APX. At the same time, cadmium stress significantly increased GSH levels, and exogenous selenium treatment further increased GSH levels, thereby increasing the tolerance of cabbage to cadmium stress. In conclusion, by protecting the photosynthetic system, eliminating excessive accumulation of reactive oxygen species in cadmium-stressed cabbage seedlings, alleviating oxidative stress and reducing cadmium levels in the plant, exogenous selenium can further improve cadmium tolerance in cabbage seedlings.
Keywords
selenium; cadmium stress; cabbage (Brassica oleracea L. var. capitata); photosynthesis; physiological characteristics
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
