Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Diagnostic Potential of MicroRNA-1, MicroRNA-21, and MicroRNA-29 as Novel Biomarkers for Early Development of Myocardial Fibrosis in Heart Failure with Preserved Ejection Fraction

Andrianto Andrianto
* ORCID logo ,
Makhyan Jibril Al-Farabi
,
Muhammad Gani Arifitrianto
,
Rahima Ratna Juwita
,
Okky Wahyu Firmansyah
,
Ryan Enast Intan
,
Asiyah Nurul Fadila
,
Muhammad Surya Tiyantara
,
I Gde Rurus Suryawan
,
Meity Ardiana
,
Nia Dyah Rahmianti
,
Budi Susetyo Pikir
,
Ricardo Adrian Nugraha
ORCID logo
Version 1 : Received: 16 August 2023 / Approved: 17 August 2023 / Online: 17 August 2023 (11:56:47 CEST)

How to cite: Andrianto, A.; Al-Farabi, M.J.; Arifitrianto, M.G.; Juwita, R.R.; Firmansyah, O.W.; Intan, R.E.; Fadila, A.N.; Tiyantara, M.S.; Suryawan, I.G.R.; Ardiana, M.; Rahmianti, N.D.; Pikir, B.S.; Nugraha, R.A. Diagnostic Potential of MicroRNA-1, MicroRNA-21, and MicroRNA-29 as Novel Biomarkers for Early Development of Myocardial Fibrosis in Heart Failure with Preserved Ejection Fraction. Preprints 2023, 2023081284. https://doi.org/10.20944/preprints202308.1284.v1 Andrianto, A.; Al-Farabi, M.J.; Arifitrianto, M.G.; Juwita, R.R.; Firmansyah, O.W.; Intan, R.E.; Fadila, A.N.; Tiyantara, M.S.; Suryawan, I.G.R.; Ardiana, M.; Rahmianti, N.D.; Pikir, B.S.; Nugraha, R.A. Diagnostic Potential of MicroRNA-1, MicroRNA-21, and MicroRNA-29 as Novel Biomarkers for Early Development of Myocardial Fibrosis in Heart Failure with Preserved Ejection Fraction. Preprints 2023, 2023081284. https://doi.org/10.20944/preprints202308.1284.v1

Abstract

This study aimed to investigate the diagnostic potential of circulating microRNAs (miRNAs) for detecting early myocardial fibrosis development in HFpEF patients. This observational study used a case-control research design. Peripheral blood samples were isolated from 25 HFpEF patients and 25 normal patients. They were measured for NTproBNP levels using ELISA, as well as MicroRNA-1, 21, and 29 levels using RT-PCR. All patients also underwent echocardiography for global longitudinal strain (GLS) to measure the myocardial fibrosis progression. Data were analyzed using SPSS 25.0. HFpEF patients had significantly higher GLS compared to the normal patients (-13±2.4% vs -19±3.2%; p < 0.05), suggesting HFpEF patients tended to have myocardial fibrosis. HFpEF patients also had significantly higher microRNA-1 and microRNA-21 compared to the normal patients (p < 0.05), but they had lower microRNA-1 (p < 0.05). There was a positive correlation between microRNA-1 (r = 0.753; p < 0.05) and microRNA-21 (r = 0.675; p < 0.05) and an inverse correlation with microRNA-29 (r = -0.653; p < 0.05) based on the GLS findings. By using a GLS cutoff of -15% for myocardial fibrosis, microRNA-1, microRNA-21, and microRNA-29 were found to be able to predict myocardial fibrosis based on GLS with a specificity of 78% and sensitivity of 75%. Increasing microRNA-1 and microRNA-21 followed by decreasing microRNA-29 in HFpEF patients suggest early myocardial fibrosis.

Keywords

diagnostic; global longitudinal strain; HFpEF, microRNA; myocardial fibrosis

Subject

Medicine and Pharmacology, Cardiac and Cardiovascular Systems

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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