Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Spin Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium-Dioxide Nanoparticles on Bovine Serum Albumin

Krisztina Sebők-Nagy
,
Zoltán Kóta
,
András Kincses
ORCID logo ,
Ákos Ferenc Fazekas
,
András Dér
ORCID logo ,
Zsuzsanna László
ORCID logo ,
Tibor Páli
*
Version 1 : Received: 11 August 2023 / Approved: 14 August 2023 / Online: 15 August 2023 (08:45:24 CEST)

A peer-reviewed article of this Preprint also exists.

Sebők-Nagy, K.; Kóta, Z.; Kincses, A.; Fazekas, Á.F.; Dér, A.; László, Z.; Páli, T. Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin. Molecules 2023, 28, 6750. Sebők-Nagy, K.; Kóta, Z.; Kincses, A.; Fazekas, Á.F.; Dér, A.; László, Z.; Páli, T. Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin. Molecules 2023, 28, 6750.

Abstract

Accumulation of proteins in filter membranes limits the efficiently of filtering technologies for cleaning wastewater. Efforts are ongoing to coat commercial filters with different materials (such as titanium-dioxide, TiO2) in order to reduce the fouling of the membrane. Beyond monitoring the desired effect of retention of biomolecules, it is demanding to understand what are the biophysical changes in water-soluble proteins caused by interacting with the new coated filter membranes, an aspect that has received little attention so far. Using spin label electron paramagnetic resonance (EPR), aided with native fluorescent spectroscopy and dynamic light scattering (DLS), here we report the changes in the structure and dynamics of bovine serum albumin (BSA) exposed to TiO2 (P25) nanoparticles or passing through commercial polyvinylidene fluoride (PVDF) membranes coated with the same nanoparticles. We have found that the filtering process and prolongued exposure to TiO2 nanoparticles had significant effects on different regions of BSA, and denaturation of the protein was not observed, neither with TiO2 nanoparticles nor by pressing through TiO2-coated filter membranes.

Keywords

wastewater cleaning; polyvinylidene fluoride filter membrane; titanium-dioxide nano-particles; P25; serum albumin; spin label EPR; fluorescence; dynamic light scattering

Subject

Environmental and Earth Sciences, Water Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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