Version 1
: Received: 9 August 2023 / Approved: 9 August 2023 / Online: 10 August 2023 (10:33:37 CEST)
How to cite:
He, Y.; Zhang, M.; Dai, C.; Yu, L. Comparison of the Gut Microbial Communities of Domestic and Wild Mallards (Anas platyrhynchos) Based on High-Throughput Sequencing Technology. Preprints2023, 2023080844. https://doi.org/10.20944/preprints202308.0844.v1
He, Y.; Zhang, M.; Dai, C.; Yu, L. Comparison of the Gut Microbial Communities of Domestic and Wild Mallards (Anas platyrhynchos) Based on High-Throughput Sequencing Technology. Preprints 2023, 2023080844. https://doi.org/10.20944/preprints202308.0844.v1
He, Y.; Zhang, M.; Dai, C.; Yu, L. Comparison of the Gut Microbial Communities of Domestic and Wild Mallards (Anas platyrhynchos) Based on High-Throughput Sequencing Technology. Preprints2023, 2023080844. https://doi.org/10.20944/preprints202308.0844.v1
APA Style
He, Y., Zhang, M., Dai, C., & Yu, L. (2023). Comparison of the Gut Microbial Communities of Domestic and Wild Mallards (<em>Anas platyrhynchos</em>) Based on High-Throughput Sequencing Technology. Preprints. https://doi.org/10.20944/preprints202308.0844.v1
Chicago/Turabian Style
He, Y., Chuanyin Dai and Lijiang Yu. 2023 "Comparison of the Gut Microbial Communities of Domestic and Wild Mallards (<em>Anas platyrhynchos</em>) Based on High-Throughput Sequencing Technology" Preprints. https://doi.org/10.20944/preprints202308.0844.v1
Abstract
Mallard (Anas platyrhynchos) are currently one of the most popular species in rare bird breeding in several southern provinces of China, but there are very few studies on the gut microbial communities of domestic and wild mallards. In this study, 16S rRNA gene high-throughput sequencing technology was used to compare the composition and diversity of gut microbial communities in domestic and wild mallards. Alpha diversity analysis revealed significant differences in the gut microbial communities of domestic and wild mallards. Beta diversity analysis showed that the two groups of stool samples were mostly separated on the principal coordinate analysis (PCOA) plot. In domestic mallards, Firmicutes (67.97%) was the most abundant bacterial phylum, followed by Proteobacteria (24.48%), Bacteroidetes (3.13%), Fusobacteria (2.21%), and Actinobacteria (1.10%). The dominant bacterial phyla in wild mallards were Firmicutes (79.02%), Proteobacteria (12.85%), Fusobacteria (3.37%), and Bacteroidetes (2.79%). At the genus level, a total of 10 dominant genera (Streptococcus, Enterococcus, Clostridium, Lactobacillus, Soilbacillus, Bacillus, Acinetobacter, Comamonas, Shigella, and Cetobacterium) with an average relative abundance greater than 1% were detected in the fecal samples of both groups. The average relative abundance of five potential pathogens (Streptococcus, Enterococcus, Acinetobacter, Comamonas, and Shigella) was higher in domestic mallards than in wild mallards. The enrichment of pathogenic bacteria in the intestinal tract of domestic mallards should be of sufficient concern.
Keywords
mallard (Anas platyrhynchos); gut microbiome; composition and diversity; 16S rRNA gene high-throughput sequencing; potential pathogens
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
