Version 1
: Received: 3 August 2023 / Approved: 4 August 2023 / Online: 4 August 2023 (09:43:23 CEST)
How to cite:
Hormozi Jangi, S.R. Time Course Studies toward Oxidation of Different Peroxidase Substrates in the Presence of Silver Nanozymes. Preprints2023, 2023080418. https://doi.org/10.20944/preprints202308.0418.v1
Hormozi Jangi, S.R. Time Course Studies toward Oxidation of Different Peroxidase Substrates in the Presence of Silver Nanozymes. Preprints 2023, 2023080418. https://doi.org/10.20944/preprints202308.0418.v1
Hormozi Jangi, S.R. Time Course Studies toward Oxidation of Different Peroxidase Substrates in the Presence of Silver Nanozymes. Preprints2023, 2023080418. https://doi.org/10.20944/preprints202308.0418.v1
APA Style
Hormozi Jangi, S.R. (2023). Time Course Studies toward Oxidation of Different Peroxidase Substrates in the Presence of Silver Nanozymes. Preprints. https://doi.org/10.20944/preprints202308.0418.v1
Chicago/Turabian Style
Hormozi Jangi, S.R. 2023 "Time Course Studies toward Oxidation of Different Peroxidase Substrates in the Presence of Silver Nanozymes" Preprints. https://doi.org/10.20944/preprints202308.0418.v1
Abstract
In this contribution, time course studies were performed toward oxidation of different peroxidase substrates in the presence of silver nanozymes. In this regard, two common peroxidase substrates, TMB and DAB, were selected as model chromogens. The process of the oxidation reaction was probed by recording the absorbance of the colored products using UV-Vis spectrophotometer. Thereafter, the nanozymatic activity was calculated and used as an index for comparing the performance of the silver nanozymes against different substrates in a certain reaction time. The time course curves, revealed that the maximal activity of the silver nanozymes were achieved after a very short time of 3.0 min toward TMB oxidation while regarding DAB, the maximal activity was observed after 25.0 min which is 6.5-fold slower than that of the TMB oxidation. The difference between the DAB and TMB can be assigned to their different reaction pathways and different reactivity. In fact, the oxidation of DAB has occurred via n-electron irreversible pathway while the TMB oxidation has occurred through a simple 2-electon reversible pathway. Besides, DAB is a high stable and less relative compound compared of TMB, resulting different oxidation times, different affinity for binding to nanozyme active nodes which lead to different nanozymatic activities.
Keywords
time-course studies; silver nanozymes; different nanozymatic activities
Subject
Chemistry and Materials Science, Biomaterials
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
