Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Breeding Substrate Containing Distillation Residues of Mediterranean Medicinal Aromatic Plants Modulates the Effects of Tenebrio molitor as Fishmeal Substitute on Blood Signal Transduction and WBC Activation of Gilthead Seabream (Sparus aurata)

Efthimia Antonopoulou
ORCID logo ,
Markos Kolygas
,
Nikolas Panteli
ORCID logo ,
Evangelia Gouva
,
Panagiota Kontogeorgiou
,
Konstantinos Feidantsis
,
Achileas Chatzopoulos
,
Konstantina Bitchava
ORCID logo ,
Christos Zacharis
,
Eleftherios Bonos
ORCID logo ,
Ιlias Giannenas
ORCID logo ,
Ioannis Skoufos
,
Stefanos S. Andreadis
ORCID logo ,
Georgios Skoulakis
,
Christos G. Athanassiou
ORCID logo ,
Cosmas Nathanailides
*
Version 1 : Received: 2 August 2023 / Approved: 3 August 2023 / Online: 3 August 2023 (10:48:40 CEST)

A peer-reviewed article of this Preprint also exists.

Antonopoulou, E.; Kolygas, M.; Panteli, N.; Gouva, E.; Kontogeorgiou, P.; Feidantsis, K.; Chatzopoulos, A.; Bitchava, K.; Zacharis, C.; Bonos, E.; Giannenas, I.; Skoufos, I.; Andreadis, S.S.; Skoulakis, G.; Athanassiou, C.G.; Nathanailides, C. Breeding Substrate Containing Distillation Residues of Mediterranean Medicinal Aromatic Plants Modulates the Effects of Tenebrio molitor as Fishmeal Substitute on Blood Signal Transduction and WBC Activation of Gilthead Seabream (Sparus aurata). Animals 2023, 13, 2537. Antonopoulou, E.; Kolygas, M.; Panteli, N.; Gouva, E.; Kontogeorgiou, P.; Feidantsis, K.; Chatzopoulos, A.; Bitchava, K.; Zacharis, C.; Bonos, E.; Giannenas, I.; Skoufos, I.; Andreadis, S.S.; Skoulakis, G.; Athanassiou, C.G.; Nathanailides, C. Breeding Substrate Containing Distillation Residues of Mediterranean Medicinal Aromatic Plants Modulates the Effects of Tenebrio molitor as Fishmeal Substitute on Blood Signal Transduction and WBC Activation of Gilthead Seabream (Sparus aurata). Animals 2023, 13, 2537.

Abstract

This work assesses the dietary use of two insect meals of Tenebrio molitor (TM) larvae reared in conventional (TM-10) or MAPs-enriched substrates (MAP-TM-10) as fish meal replacements (10%) in the diets of gilthead seabream (Sparus aurata). Fish (n=4,500; 207.19 ± 1.47 g) were divided into three groups with triplicates: Control (fed conventional diet), TM-10 and MAP-TM-10 groups.The fish were reared in floating cages for 12 weeks and The dietary effects on white blood cell ac-tivation, heat shock proteins, MAPKs, and apoptosis of the fish were evaluated. The MAP-TM-10 group exhibited the highest eosinophilic induction and plasma cells. Phosphorylated levels of p38 MAPK; p44/42 MAPK; HSP70 and HSP90 increased in the TM-10 and MAP-TM-10. In terms of apoptosis, Bax levels were lower in the TM groups compared to the Control, and the MAP-TM-10 group showed even lower levels than the TM-10 group. Bcl-2 levels increased in the TM-10 compared to the Control, and further increased in the MAP-TM-10 group. The Bax/Bcl-2 ratio, an apoptosis indicator, decreased in the TM groups, with the MAP-TM-10 group showing a further decrease compared to TM-10. These findings suggest that insects breeding substrate enriched with MAPs modulated the effect of TM on cellular stress and apoptosis.

Keywords

Tenebrio molitor; gilthead sea bream; differential leukocyte count; MAPK signaling; heat shock proteins

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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