Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Influence of Encapsulation of Propolis Extract with 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) on the Polyphenolic Contents during In Vitro Simulation of Digestion

Eleonora Perak Junaković
ORCID logo ,
Ksenija Šandor
* ORCID logo ,
Svjetlana Terzić
,
Anja Vujnović
,
Miroslav Andrišić
,
Miroslav Benić
,
Dominika Fajdić
,
Sonja Sinković
,
Mirta Pehnec
,
Irena Žarković
ORCID logo
Version 1 : Received: 27 July 2023 / Approved: 27 July 2023 / Online: 28 July 2023 (14:00:26 CEST)

A peer-reviewed article of this Preprint also exists.

Perak Junaković, E.; Šandor, K.; Terzić, S.; Vujnović, A.; Andrišić, M.; Benić, M.; Fajdić, D.; Sinković, S.; Pehnec, M.; Žarković, I. Influence of Encapsulation of Propolis Extract with 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) on Polyphenolic Contents during In Vitro Simulation of Digestion. Appl. Sci. 2023, 13, 9357. Perak Junaković, E.; Šandor, K.; Terzić, S.; Vujnović, A.; Andrišić, M.; Benić, M.; Fajdić, D.; Sinković, S.; Pehnec, M.; Žarković, I. Influence of Encapsulation of Propolis Extract with 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) on Polyphenolic Contents during In Vitro Simulation of Digestion. Appl. Sci. 2023, 13, 9357.

Abstract

The purpose of this investigation was to prepare a water-soluble complex by encapsulation of propolis extract with the carrier 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and to examine the influence of in vitro simulated gastrointestinal digestion (GID) on the content of the main polyphenolic groups when administered as aqueous suspension. The research was conducted on two continental samples of propolis from Croatia and a mixture of selected polyphenol standards. By applying UAE followed by a lyophilization procedure, it was possible to obtain a powder with a high content of polyphenols. The content of total phenols (TPs), total flavonols/flavones (TFFs) and total flavanones/dihydroflavonols (TFDs) in the fractions of oral and gastric phase and small intestinal phase performed with two models: centrifugation and dialysis through a semi-permeable membrane, was determined by three UV/Vis spectrophotometric methods: Folin-Ciocalteu, aluminium complexation and 2,4-dinitrophenylhydrazine method. The obtained results for both propolis complexes showed stability in the polyphenol content and high bioaccessibility during all three phases of digestion. The bioaccessibility of TPs, TFFs and TFDs under in vitro conditions after dialysis through a semipermeable membrane was lower compared to the intestinal phase model with centrifugation.

Keywords

poplar propolis; phenolics; flavones; flavonols; flavanones; cyclic oligosaccharide; UV/VIS spectrophotometry; gastrointestinal digestion; bioaccessibility

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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