Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Identification of Delta Variant-Specific Mutations of SARS-CoV-2 Genomes Found in Uzbek Patients by Amplicon Sequencing

Mirzakamol Ayubov
* ORCID logo ,
Zabardast Buriev
,
Mukhammadjon Mirzakhmedov
,
Abdurakhmon Yusupov
,
Bakhtiyor Nosirov
,
Dilshod Usmanov
,
Shukhrat Shermatov
,
Khurshida Ubaydullaeva
,
Abdusattor Abdukarimov
,
Ibrokhim Abdurakhmonov
* ORCID logo
Version 1 : Received: 25 July 2023 / Approved: 26 July 2023 / Online: 27 July 2023 (09:36:12 CEST)

How to cite: Ayubov, M.; Buriev, Z.; Mirzakhmedov, M.; Yusupov, A.; Nosirov, B.; Usmanov, D.; Shermatov, S.; Ubaydullaeva, K.; Abdukarimov, A.; Abdurakhmonov, I. Identification of Delta Variant-Specific Mutations of SARS-CoV-2 Genomes Found in Uzbek Patients by Amplicon Sequencing. Preprints 2023, 2023071814. https://doi.org/10.20944/preprints202307.1814.v1 Ayubov, M.; Buriev, Z.; Mirzakhmedov, M.; Yusupov, A.; Nosirov, B.; Usmanov, D.; Shermatov, S.; Ubaydullaeva, K.; Abdukarimov, A.; Abdurakhmonov, I. Identification of Delta Variant-Specific Mutations of SARS-CoV-2 Genomes Found in Uzbek Patients by Amplicon Sequencing. Preprints 2023, 2023071814. https://doi.org/10.20944/preprints202307.1814.v1

Abstract

The rapid changes in the coronavirus genomes are creating new strains over time after the first variation found in Wuhan in 2019. SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) genotypes need periodically undergo whole genome sequencing. Genome sequencing has been extremely helpful in combating this virus because so many diagnoses, treatments, and vaccinations have been developed against it. To achieve this, we generated 17 high-quality whole-genome sequence data from 48 SARS-CoV-2 genotypes of COVID-19 patients who tested positive by PCR in the Tashkent city of Uzbekistan. We identified nucleotide polymorphisms, including nonsynonymous (missense) and synonymous mutations in the coding regions of the sequenced sample genomes. All whole genome sequences were categorized by phylogenetic analysis into the G clade (or GK sub-clade). A total of 134 mutations were identified, consisting of 65 shared and 69 unique mutations. Collectively, nucleotide changes represented one frameshift mutation, one conservative and disruptive insertion deletion, four upstream region mutations, four downstream region mutations, 39 synonymous mutations, and 84 missense mutations. Furthermore, bioinformatics web tools were used to analyze amino acid changes in virus genomes. Several amino acid mutations were found, which were not found in previously published Delta strain sequences.

Keywords

SARS-CoV-2; virus; transmission; mutations; Delta strain

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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