Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Inhibition of Trichinella spiralis Membrane‐Associated Progesterone Receptor (MAPR) Results in the Reduction of Worm Burden

Muhammad Tahir Aleem
ORCID logo ,
Zhaohai Wen
,
Zhengqing Yu
ORCID logo ,
Cheng Chen
,
Mingmin Lu
ORCID logo ,
Lixin Xu
ORCID logo ,
Xiaokai Song
,
Xiangrui Li
ORCID logo ,
Ruofeng Yan
*
Version 1 : Received: 24 July 2023 / Approved: 24 July 2023 / Online: 24 July 2023 (11:47:59 CEST)

A peer-reviewed article of this Preprint also exists.

Aleem, M.T.; Wen, Z.; Yu, Z.; Chen, C.; Lu, M.; Xu, L.; Song, X.; Li, X.; Yan, R. Inhibition of Trichinella spiralis Membrane-Associated Progesterone Receptor (MAPR) Results in a Reduction in Worm Burden. Vaccines 2023, 11, 1437. Aleem, M.T.; Wen, Z.; Yu, Z.; Chen, C.; Lu, M.; Xu, L.; Song, X.; Li, X.; Yan, R. Inhibition of Trichinella spiralis Membrane-Associated Progesterone Receptor (MAPR) Results in a Reduction in Worm Burden. Vaccines 2023, 11, 1437.

Abstract

Trichinella spiralis (T. spiralis), a nematode parasite, is the major cause of Trichinellosis, a zoonotic disease. A key role of MAPR in the reproductive system is to maintain pregnancy. Previous studies found antihormone (P4 and RU486) drug design and vaccine therapy of recombinant protein (rTs-MAPRC2) to control T. spiralis infection. The current study investigates the inhibitory effects of different ratios of antibody against Ts-MAPRC2 on the development of muscle larvae (ML) and newborn larvae (NBL). First, we performed indirect immunofluorescence assays (IIFA) and examined the effects of rTs-MAPRC2-Ab on ML and NBL in vitro as well as in vivo. After-ward, siRNA-Ts-MAPRC2 was transfected into T. spiralis muscle larvae. Following that, Ts-MAPRC2 protein was detected by Western Blotting and mRNA levels were determined by qPCR. Also, we assessed whether siRNA-treated NBLs were infective by analyzing muscle larvae burden (MLs). Our Result showed rTs-MAPRC2-Ab greatly inhibited the activity of the Ts-MAPRC2 gene in ML and NBL of T. spiralis. rTs-MAPRC2-Ab reduced larval infectivity and survival in the host in a dose-dependent manner (1:50, 1:200, 1:800 dilutions). Further, siR-NA-Ts-MAPRC2 effectively silenced the Ts-MAPRC2 gene in muscle larvae (ML) by in vitro, as well as in new-born larvae (NBL) of T. spiralis by in vivo. In addition, siRNA-Ts-MAPRC2 (siR-NA180, siRNA419, siRNA559) reduced host larval survival and infectivity significantly. The effect of siRNA on larval development, survival, and infectivity was significantly reduced when the Ts-MAPRC2 gene was knocked down. This study, therefore, suggests that Ts-MAPRC2 might be a novel molecular target that might be useful in the development of vaccines against T. spiralis infection.

Keywords

inhibitory effect; knockdown; rTs‐MAPRC2‐Ab; Trichinella spiralis; worm burden

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.