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Elucidating the Anti‐Obesity Potential of Bioactive Fractions of Kalanchoe pinnata (Lam.) Leaves Extract Using a Combination of In Vitro, In Vivo and In Silico Methods Along with Characterization of Lead Compounds through an HPTLC MS‐MSn Analytical Study
Version 1
: Received: 20 July 2023 / Approved: 21 July 2023 / Online: 24 July 2023 (03:29:44 CEST)
How to cite:
Singh, R.P.; Pattnaik, A.K.; Rudrapal, M. Elucidating the Anti‐Obesity Potential of Bioactive Fractions of Kalanchoe pinnata (Lam.) Leaves Extract Using a Combination of In Vitro, In Vivo and In Silico Methods Along with Characterization of Lead Compounds through an HPTLC MS‐MSn Analytical Study. Preprints2023, 2023071472. https://doi.org/10.20944/preprints202307.1472.v1
Singh, R.P.; Pattnaik, A.K.; Rudrapal, M. Elucidating the Anti‐Obesity Potential of Bioactive Fractions of Kalanchoe pinnata (Lam.) Leaves Extract Using a Combination of In Vitro, In Vivo and In Silico Methods Along with Characterization of Lead Compounds through an HPTLC MS‐MSn Analytical Study. Preprints 2023, 2023071472. https://doi.org/10.20944/preprints202307.1472.v1
Singh, R.P.; Pattnaik, A.K.; Rudrapal, M. Elucidating the Anti‐Obesity Potential of Bioactive Fractions of Kalanchoe pinnata (Lam.) Leaves Extract Using a Combination of In Vitro, In Vivo and In Silico Methods Along with Characterization of Lead Compounds through an HPTLC MS‐MSn Analytical Study. Preprints2023, 2023071472. https://doi.org/10.20944/preprints202307.1472.v1
APA Style
Singh, R.P., Pattnaik, A.K., & Rudrapal, M. (2023). Elucidating the Anti‐Obesity Potential of Bioactive Fractions of Kalanchoe pinnata (Lam.) Leaves Extract Using a Combination of In Vitro, In Vivo and In Silico Methods Along with Characterization of Lead Compounds through an HPTLC MS‐MSn Analytical Study. Preprints. https://doi.org/10.20944/preprints202307.1472.v1
Chicago/Turabian Style
Singh, R.P., Ashok Kumar Pattnaik and Mithun Rudrapal. 2023 "Elucidating the Anti‐Obesity Potential of Bioactive Fractions of Kalanchoe pinnata (Lam.) Leaves Extract Using a Combination of In Vitro, In Vivo and In Silico Methods Along with Characterization of Lead Compounds through an HPTLC MS‐MSn Analytical Study" Preprints. https://doi.org/10.20944/preprints202307.1472.v1
Abstract
Bioactive fractions were isolated from the leaves extract of Kalanchoe pinnata and subjected to scrutiny for their prospective anti-obesity properties. An array of in vitro phytochemical, antioxidant, and enzyme inhibition assays were executed, which dis-cerned fractions F1 and F2 as the most effective fractions. These fractions were sub-sequently studied through in vivo experiments, affirming that F2 as the most potent fraction. Further characterization of F2 was conducted via HPTLC MS-MSn tech-niques. The outcomes demonstrated that F2 produced a notable anti-obesity effect in obese mice, reducing their body weight and lipid metrics, and leading to advanta-geous changes in their organs. An analytical examination of F2 revealed the existence of four principal compounds, which were subsequently subjected to in silco molecular docking and dynamic analysis, confirming their aptitude for binding to selected pro-teins. These findings imply that the utilization of Kalanchoe pinnata leaves could pro-vide a promising therapeutic strategy for the treatment of obesity.
Keywords
anti‐obesity; bio‐active fractions; Kalanchoe pinnata; HPTLC MS‐MSn; in silico; in vitro and in vivo studies
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
