Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Double Negative B lymphocytes are increased in patients with Systemic Lupus Erythematosus and they are correlated with early differentiated T lymphocyte subpopulations

Eleni Moysidou
ORCID logo ,
Georgios Lioulios
,
Michalis Christodoulou
ORCID logo ,
Aliki Xochelli
,
Stamatia Stai
,
Myrto Iosifidou
,
Artemis Iosifidou
,
Sophia Briza
,
Dimitria Ioanna Briza
,
Asimina Fylaktou
,
Maria Stangou
* ORCID logo
Version 1 : Received: 7 July 2023 / Approved: 10 July 2023 / Online: 10 July 2023 (10:33:26 CEST)

A peer-reviewed article of this Preprint also exists.

Moysidou, E.; Lioulios, G.; Christodoulou, M.; Xochelli, A.; Stai, S.; Iosifidou, M.; Iosifidou, A.; Briza, S.; Briza, D.I.; Fylaktou, A.; Stangou, M. Increase in Double Negative B Lymphocytes in Patients with Systemic Lupus Erythematosus in Remission and Their Correlation with Early Differentiated T Lymphocyte Subpopulations. Curr. Issues Mol. Biol. 2023, 45, 6667-6681. Moysidou, E.; Lioulios, G.; Christodoulou, M.; Xochelli, A.; Stai, S.; Iosifidou, M.; Iosifidou, A.; Briza, S.; Briza, D.I.; Fylaktou, A.; Stangou, M. Increase in Double Negative B Lymphocytes in Patients with Systemic Lupus Erythematosus in Remission and Their Correlation with Early Differentiated T Lymphocyte Subpopulations. Curr. Issues Mol. Biol. 2023, 45, 6667-6681.

Abstract

B and T lymphocytes demonstrate important alterations in patients with Systemic Lupus Erythematous (SLE), with significant up-regulation of Double Negative (DN) B cells. Aim of this study was to evaluate correlation of T cell immunity changes with the distinct B cell-pattern SLE. In the present study, Flow cytometry was performed in 30 patients with SLE, on remission, and 31 healthy controls, to detect DN B cells (CD19+IgD-CD27-) and a wide range of T lymphocyte subpopulations, based on the presence of CD45RA, CCR7, CD31, CD28, CD57, defined as naive, memory and advanced differentiated/senescent T cells. Both B and T lymphocytes were significantly reduced in SLE patients. However, the percentage of DN B cells were increased, compared to HC, 12.9(2.3-74.2) vs. 8(1.7-35), p=0.04. Distribution of CD4 and CD8 lymphocytes demonstrated a shift to advanced differentiated subsets. The population of DN B cells had significant positive correlation with most of early differentiated T lymphocytes, CD4CD31+, CD4CD45RA+CD28+, CD4CD45RA+CD57-, CD4CD45RA-CD57-, CD4CD28+CD57-, CD4CD28+CD57+, CD4 CM, CD8 CD31+, CD8 NAÏVE, CD8CD45RA-CD57-, CD8CD28+CD57-, CD8CD28+CD57+. Multiple Regression analysis revealed CD4CD31+, CD8CD45RA-CD57- and CD8CD28+CD57- cells as independent parameters contributing to DN B cells, adjusted R2=0.534, p<0.0001. The predominance of DN B cells in patients with SLE is closely associated with early differentiated T lymphocyte subsets, indicating a potential causality role of DN B cells in T lymphocyte activation.

Keywords

Systemic Lupus Erythematosus; Lupus nephritis; Double negative B lymphocytes; T lymphocytes; early differentiated cells; senescent lymphocytes

Subject

Medicine and Pharmacology, Urology and Nephrology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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