Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Obtaining of New Antioxidant and Antimicrobial Peptides Derived from Human Hemoglobin by Peptide Hydrolysis and Comparison with These Obtained by Bovine Hemoglobin

Ahlam Outman
,
Barbara Deracinois
ORCID logo ,
Christophe Flahaut
ORCID logo ,
Mira Abou Diab
,
Jihen Dhaouefi
,
Bernard Gressier
,
Bruno Eto
* ,
Naima Nedjar
Version 1 : Received: 5 July 2023 / Approved: 5 July 2023 / Online: 6 July 2023 (02:12:27 CEST)

A peer-reviewed article of this Preprint also exists.

Outman, A.; Deracinois, B.; Flahaut, C.; Diab, M.A.; Dhaouefi, J.; Gressier, B.; Eto, B.; Nedjar, N. Comparison of the Bioactive Properties of Human and Bovine Hemoglobin Hydrolysates Obtained by Enzymatic Hydrolysis: Antimicrobial and Antioxidant Potential of the Active Peptide α137-141. Int. J. Mol. Sci. 2023, 24, 13055. Outman, A.; Deracinois, B.; Flahaut, C.; Diab, M.A.; Dhaouefi, J.; Gressier, B.; Eto, B.; Nedjar, N. Comparison of the Bioactive Properties of Human and Bovine Hemoglobin Hydrolysates Obtained by Enzymatic Hydrolysis: Antimicrobial and Antioxidant Potential of the Active Peptide α137-141. Int. J. Mol. Sci. 2023, 24, 13055.

Abstract

This study focuses on the enzymatic hydrolysis of hemoglobin, the main component of cruor that gives blood its red color in mammals. The antibacterial and antioxidant potential of human hemoglobin hydrolysates were evaluated in comparison to bovine hemoglobin. The results showed strong antimicrobial activity of the peptide hydrolysates against six bacterial strains, independent of the initial substrate concentration level. The hydrolysates also showed strong antioxidant activity measured by four different tests. In addition, the antimicrobial and antioxidant activity of the human and bovine hemoglobin hydrolysates showed little or no significant difference, with only the concentration level being the determining factor in their activity. The results of the mass spectrometry study showed the presence of a number of bioactive peptides, the majority of which have characteristics similar to those mentioned in the literature. New bioactive peptides were also identified in human hemoglobin, such as antibacterial peptides PTTKTYFPHF (α37-46), FPTTKTYFPH (α36-45), TSKYR (α137-141) and STVLTSKYR (α133-141), as well as an antioxidant TSKYR (α137-141). According to these findings, human hemoglobin represents a promising source of bioactive peptides beneficial to the food or pharmaceutical industries.

Keywords

human hemoglobin; bovine hemoglobin; bioactive peptides; antibacterial activity; antioxidant activity

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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