Park, J.; Nguyen, T.M.N.; Park, H.-A.; Nguyen, M.T.T.; Lee, N.-Y.; Ban, S.-Y.; Park, K.-B.; Lee, C.-K.; Kim, J.; Park, J.-T. Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses. Int. J. Mol. Sci.2023, 24, 12803.
Park, J.; Nguyen, T.M.N.; Park, H.-A.; Nguyen, M.T.T.; Lee, N.-Y.; Ban, S.-Y.; Park, K.-B.; Lee, C.-K.; Kim, J.; Park, J.-T. Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses. Int. J. Mol. Sci. 2023, 24, 12803.
Park, J.; Nguyen, T.M.N.; Park, H.-A.; Nguyen, M.T.T.; Lee, N.-Y.; Ban, S.-Y.; Park, K.-B.; Lee, C.-K.; Kim, J.; Park, J.-T. Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses. Int. J. Mol. Sci.2023, 24, 12803.
Park, J.; Nguyen, T.M.N.; Park, H.-A.; Nguyen, M.T.T.; Lee, N.-Y.; Ban, S.-Y.; Park, K.-B.; Lee, C.-K.; Kim, J.; Park, J.-T. Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses. Int. J. Mol. Sci. 2023, 24, 12803.
Abstract
Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushroom have been investigated for various allergic diseases. However, antiaging and anti-inflammatory activities of inotodiol under different types of oxidative stress and impact of inotodiol on collagen and hyaluronan synthesis are not sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (the inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulation such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced overexpressions of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role of pure inotodiol/inotodiol concentrate in prevention of skin aging. These results enlighten anti-inflammatory and anti-aging effect of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.
Medicine and Pharmacology, Medicine and Pharmacology
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