Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6) Promotes Metastatic Spread to the Lung in Advanced Prostate Cancer

Alireza Saraji
ORCID logo ,
Katharina Wulf
,
Janine Stegmann-Frehse
,
Duan Kang
,
Anne Offermann
,
Juta Kirfel
,
Sven Perner
,
Verena Wilbeth Sailer
*
Version 1 : Received: 30 June 2023 / Approved: 30 June 2023 / Online: 30 June 2023 (10:46:51 CEST)

How to cite: Saraji, A.; Wulf, K.; Stegmann-Frehse, J.; Kang, D.; Offermann, A.; Kirfel, J.; Perner, S.; Sailer, V.W. Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6) Promotes Metastatic Spread to the Lung in Advanced Prostate Cancer. Preprints 2023, 2023062221. https://doi.org/10.20944/preprints202306.2221.v1 Saraji, A.; Wulf, K.; Stegmann-Frehse, J.; Kang, D.; Offermann, A.; Kirfel, J.; Perner, S.; Sailer, V.W. Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6) Promotes Metastatic Spread to the Lung in Advanced Prostate Cancer. Preprints 2023, 2023062221. https://doi.org/10.20944/preprints202306.2221.v1

Abstract

Prostate cancer (PCa) Lung metastases are rarely resected, therefore PCa lung metastases are insufficiently molecularly characterized. We recently identified carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) as a potential driver of pulmonary metastatic spread. Here, we show the biological significance of CEACAM6 in PCa cell proliferation, apoptosis and migration. CEACAM6 was silenced by siRNA in PC3 cells. Functional assessment of apoptosis, cell viability, proliferation and migration were performed in siRNA-CEACAM6 PC3 cells. Non-treated and scrambled (scr) RNA PC3 cells were used as control. Following a specific knockdown of CEACAM6 in PC3 cells, the expression of CEACAM6 protein was significantly decreased in comparison to controls. Cell viability and cell counts decreased in CEACAM6 silent PC3 cells. In contrast, caspase-3 activity was highly elevated in siRNA-CEACAM6 PC3. Furthermore, by performing a cell scratch assay, the migration ratio in siRNA-CEACAM6 PC3 cells were significantly diminished compared with the control group after 48 hours of post transfection incubation. CEACAM6 as a cell adhesion molecule has been implicated in promoting metastatic disease in several solid tumours such as colorectal or gastric cancer. We could show that silencing of CEACAM6 has a significant functional effect on PCa cells. CEACAM6 might play an important role in fostering metastatic spread to the lung of PCa patients via enhancing proliferation and suppressing apoptosis. CEACAM6 might therefore pose an attractive therapeutic target to prevent metastatic disease.

Keywords

prostate cancer; microenvironment; lung metastases; gene expression

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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