Mapping and Verification of QTL for Grain Shape and Grain Chalkiness in Rice Using Sequencing Technology

Yingying Wang; Wenhan Dan; Huan Shi; Mingsong Zhou; Hao Zhou; Duo Xia; Qinglu Zhang; Guanjun Gao; Yuqing He

doi:10.20944/preprints202306.2072.v1

Comment 1

Received: 30 June 2023
Commenter:

Yuqing He

Commenter's Conflict of Interests: I am one of the author

Comment: Rice is one of the major crops and provide more than 21% calorie for the world population. In the past decades, breeders are increasingly paying attention to rice quality alone with the improvement of rice yield. As a lower quality trait, rice chalkiness affects not only appearance but also processing and cooking characteristics. Till now, a number of major genes were cloned and charactered to affect grain size, however, minor genes are also an important component in determining grain size and can be used to clarify the molecular mechanism that controlling grain size. Besides, only a few gene were found influenced rice chalkiness and successfully cloned. Therefore, it is necessary to discover more genetic resources that can decrease rice chalkiness for the breeding and improvement of rice varieties.
In this study, the genetic map of F2 population was conducted by using sequencing technology, and genetic linkage analysis of grain shape and chalkiness related traits was performed. A total of 15 QTL affect rice grain shape and chalkiness related traits were detected in F2 and F2:3 populations. A QTL cluster affecting multiple chalkiness traits was found on chromosome 6, qWBR6.1, qWCR6 and qCR6 were selected for genetic effect verification. qWBR6.1, qWCR6 and qCR6 were found explained 13% and 24%, and 29% of the phenotypic variations respectively, and qWCR6 and qCR6 were co-mapped. This study laid a foundation for further cloning of rice appearance quality genes.

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Mapping and Verification of QTL for Grain Shape and Grain Chalkiness in Rice Using Sequencing Technology

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