Narcizo, J.P.; Mancilio, L.B.K.; Pedrino, M.; Guazzaroni, M.-E.; de Andrade, A.R.; Reginatto, V. A New Pseudomonas aeruginosa Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System. Catalysts2023, 13, 1133.
Narcizo, J.P.; Mancilio, L.B.K.; Pedrino, M.; Guazzaroni, M.-E.; de Andrade, A.R.; Reginatto, V. A New Pseudomonas aeruginosa Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System. Catalysts 2023, 13, 1133.
Narcizo, J.P.; Mancilio, L.B.K.; Pedrino, M.; Guazzaroni, M.-E.; de Andrade, A.R.; Reginatto, V. A New Pseudomonas aeruginosa Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System. Catalysts2023, 13, 1133.
Narcizo, J.P.; Mancilio, L.B.K.; Pedrino, M.; Guazzaroni, M.-E.; de Andrade, A.R.; Reginatto, V. A New Pseudomonas aeruginosa Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System. Catalysts 2023, 13, 1133.
Abstract
The ability of some bacteria to perform Extracellular Electron Transfer (EET) has been explored in bioelectrochemical systems (BES) to obtain energy or chemicals from pure substances or residual substrates. Here, a new pyoverdine-producing Pseudomonas aeruginosa strain was isolated from a MFC biofilm oxidizing glycerol, a by-product of biodiesel production. Strain EL14 was investigated to assess its electrogenic ability and products. In an open circuit system (fermentation system) EL14 was able to consume glycerol and produce 1,3-propanediol, an unusual product from glycerol oxidation in P. aeruginosa. The microbial fuel cell (MFC), EL14 reached a current density of 82.4 mA m-2, during the first feeding cycle, then drops sharply as the biofilm falls off. Cyclic voltammetry suggests electron transfer to the anode occurrs indirectly, i.e., through a redox substance, with redox peaks at 0.22 and -0.45 V (vs Ag/AgCl) and directly probably by membrane redox-proteins with redox peak at 0.05 V (vs Ag/AgCl). EL14 produced added-value bioproducts, acetic and butyric acids, as well as 1,3 propanediol, in both fermentative and anodic conditions. However, the yield of 1,3-PDO from glycerol was enhanced from 0.57 to 0.89 (mol of 1,3-PDO mol-1 of glycerol) under MFC conditions compared with the fermentation. This result was unexpected since successful 1,3-PDO production is not usually associated with the P. aeruginosa glycerol metabolism. By comparing EL14 genomic sequences related to the 1,3-PDO biosynthesis with reference P. aeruginosa strains, we observed that strain EL14 has three copies of dhaT gene (1,3-propanediol dehydrogenase a different arrangement compared to other Pseudomonas isolates). Thus, this work functionally characterizes a bacterium never before associated to 1,3-PDO biosynthesis, indicating its potential for converting a by-product of the biodiesel industry into an emerging chemical product.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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