Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biofilm Adhesion and Micromorphology Analysis After Professional Oral Hygiene Procedures on Cad/Cam Milled and Veneered Zirconia Restorations – In Vitro Study

Mirjana Perić
ORCID logo ,
Aleksandra Čairović
* ORCID logo ,
Aleksandra Špadijer Gostović
,
Dragan Stanimirović
,
Marina Kostić
ORCID logo ,
Marija Ivanov
ORCID logo ,
Ana Ćirić
ORCID logo ,
Jasmina Glamocija
ORCID logo ,
Vladimir B. Pavlovic
Version 1 : Received: 23 June 2023 / Approved: 25 June 2023 / Online: 25 June 2023 (07:13:11 CEST)

A peer-reviewed article of this Preprint also exists.

Perić, M.; Čairović, A.; Špadijer Gostović, A.; Stanimirović, D.; Kostić, M.; Ivanov, M.; Ćirić, A.; Glamočlija, J.; Pavlović, V.B. Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. Appl. Sci. 2023, 13, 8389. Perić, M.; Čairović, A.; Špadijer Gostović, A.; Stanimirović, D.; Kostić, M.; Ivanov, M.; Ćirić, A.; Glamočlija, J.; Pavlović, V.B. Biofilm Adhesion and Micromorphology Analysis after Professional Oral Hygiene Procedures on CAD/CAM Milled and Veneered Zirconia Restorations: In Vitro Study. Appl. Sci. 2023, 13, 8389.

Abstract

Objective: The purpose of this study was to evaluate the surface texture and biofilm adhesion of veneered or CAD/CAM milled zirconia (partially stabilized with yttrium) after professional oral hygiene procedures. Samples (4x4 mm, thickness 2 mm; n = 72) were separated from zirconia blanks (3Y-TZP-LA). One group was veenired with ceramics and the other group of samples was CAD/CAM milled. Each group had 2 subgroups: polished and glazed. The samples were subjected to simulated strokes of professional brushing using abrasive paste and ultrasonic scaling. Parameters of surface micromorphology and receptivity to biofilm were calculated before and after simulating the given methods of professional maintenance of oral hygiene. The characteristics of zirconia surface were evaluated by scanning electron microscopy (SEM). Microbial bacterial/fungal species (Staphylococcus aureus, Streptococcus sanguinis and Candida albicans) were used and cultured on the respective sterilized zirconia surfaces. The amount of biofilm formation on zirconia surface was quantified by colony forming unit (CFU) counts. Results: SEM analysis showed the greatest change in surface microtopography after the use of ultrasonic scaling, in glazed zirconia samples. Less formation of colonies on the surface CAD/CAM milled zirconia restorations was observed. Conclusion: Routine methods of professional maintenance of oral hygiene can damage the surface of glazed zirconia restorations.

Keywords

Zirconia; CAD/CAM; ultrasonic scaling; SEM; surface micro topography; microbial biofilm;

Subject

Chemistry and Materials Science, Ceramics and Composites

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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