Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

CfDNA and Caspase-3 Levels in Patients with Chronic Kidney Disease

Anna Clementi
* ,
Grazia Maria Virzì
* ORCID logo ,
Sabrina Milan Manani
,
Massimo De Cal
ORCID logo ,
Giovanni Giorgio Battaglia
,
Claudio Ronco
,
Monica Zanella
*
Equally contributed.
Version 1 : Received: 15 June 2023 / Approved: 16 June 2023 / Online: 16 June 2023 (03:31:05 CEST)

A peer-reviewed article of this Preprint also exists.

Clementi, A.; Virzì, G.M.; Manani, S.M.; de Cal, M.; Battaglia, G.G.; Ronco, C.; Zanella, M. Plasma Cell-Free DNA and Caspase-3 Levels in Patients with Chronic Kidney Disease. J. Clin. Med. 2023, 12, 5616. Clementi, A.; Virzì, G.M.; Manani, S.M.; de Cal, M.; Battaglia, G.G.; Ronco, C.; Zanella, M. Plasma Cell-Free DNA and Caspase-3 Levels in Patients with Chronic Kidney Disease. J. Clin. Med. 2023, 12, 5616.

Abstract

Background: Cell-free plasma DNA (cfDNA) is composed of circulating extracellular DNA originating from apoptosis or cell necrosis. It is generally present in healthy individuals, but its levels increase in different clinical conditions, such as kidney disease, sepsis, myocardial infarction, trauma and cancer. Caspase-3 plays a central role in apoptosis, a mechanism of programmed cell death involved in the pathogenesis of chronic kidney disease (CKD). Methods: We compared cfDNA and caspase-3 levels in 25 CKD patients and in 10 healthy subjects, evaluating the modification of their concentrations according to CKD stage. We also analyzed the correlation between cfDNA and caspase-3 levels in CKD patients and the correlation between cfDNA and caspase-3 levels and creatinine and urea. Results: CfDNA and caspase-3 levels were higher in CKD patients compared to healthy subjects, in particular in patients with advanced renal disease. A positive correlation between cfDNA and caspase-3 levels and between cfDNA and caspase-3 and creatinine and urea was also observed. Conclusion: Patients with chronic kidney disease present higher levels of cfDNA and caspase-3 levels compared with healthy subjects. The worsening of renal damage and the increase in uremic toxin concentration are associated to higher levels of cfDNA and caspase-3 levels, thus reflecting a central role of apoptosis in the pathogenesis of CKD.

Keywords

cell free DNA, caspase-3, chronic kidney disease,

Subject

Medicine and Pharmacology, Clinical Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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