Ezawa, N.; Akashi, Y.; Nakajima, K.; Kokubun, K.; Furusawa, M.; Matsuzaka, K. The Effects of Tricalcium-Silicate-Nanoparticle-Containing Cement: In Vitro and In Vivo Studies. Materials2023, 16, 4451.
Ezawa, N.; Akashi, Y.; Nakajima, K.; Kokubun, K.; Furusawa, M.; Matsuzaka, K. The Effects of Tricalcium-Silicate-Nanoparticle-Containing Cement: In Vitro and In Vivo Studies. Materials 2023, 16, 4451.
Ezawa, N.; Akashi, Y.; Nakajima, K.; Kokubun, K.; Furusawa, M.; Matsuzaka, K. The Effects of Tricalcium-Silicate-Nanoparticle-Containing Cement: In Vitro and In Vivo Studies. Materials2023, 16, 4451.
Ezawa, N.; Akashi, Y.; Nakajima, K.; Kokubun, K.; Furusawa, M.; Matsuzaka, K. The Effects of Tricalcium-Silicate-Nanoparticle-Containing Cement: In Vitro and In Vivo Studies. Materials 2023, 16, 4451.
Abstract
A tricalcium silicate nanoparticle-containing cement (NPC) was developed to overcome the disadvantages of existing mineral trioxide aggregate (MTA) dental materials. This study aimed at evaluating the effects of NPC on the osteogenic differentiation of human periodontal ligament fibroblasts (HPLFs) in vitro, and on the healing of furcal perforations created experimentally in rat molars in vivo, in comparison to MTA. The in vitro studies performed the following assays; pH measurement using a pH meter, the release of calcium ions using a calcium assay kit, cell attachment and morphology using SEM, cell proliferation using a coulter counter, marker expression using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and cell mineralized deposit formation using Alizarin Red S (ARS) staining. In the in vivo studies, MTA and NPC were used to fill the rat molar perforations. Rat molars were processed at 7, 14 and 28 days for analysis of inflammatory processes using hematoxylin and eosin (HE) staining, immunohistochemical staining of Runx2 and tartrate-resistant acid phosphate (TRAP) staining. The results demonstrate that the nanoparticle size distribution of NPC is critical for osteogenic potential at an earlier stage compared to MTA. Further studies are required to elucidate the mechanism of action of NPC in osteogenic differentiation.
Keywords
tricalcium silicate nanoparticle-containing cement (NPC); mineral trioxide aggregate (MTA); human periodontal ligament fibroblasts (HPLFs)
Subject
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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