Akbayeva, D.N.; Smagulova, I.A.; Maksotova, K.S.; Bakirova, B.S.; Tatykhanova, G.S.; Kudaibergenov, S.E. In Situ Entrapment of Catalase within Macroporous Cryogel Matrix for Ethanol Oxidation: Flow-through Mode versus Batch Reactor. Catalysts2023, 13, 1075.
Akbayeva, D.N.; Smagulova, I.A.; Maksotova, K.S.; Bakirova, B.S.; Tatykhanova, G.S.; Kudaibergenov, S.E. In Situ Entrapment of Catalase within Macroporous Cryogel Matrix for Ethanol Oxidation: Flow-through Mode versus Batch Reactor. Catalysts 2023, 13, 1075.
Akbayeva, D.N.; Smagulova, I.A.; Maksotova, K.S.; Bakirova, B.S.; Tatykhanova, G.S.; Kudaibergenov, S.E. In Situ Entrapment of Catalase within Macroporous Cryogel Matrix for Ethanol Oxidation: Flow-through Mode versus Batch Reactor. Catalysts2023, 13, 1075.
Akbayeva, D.N.; Smagulova, I.A.; Maksotova, K.S.; Bakirova, B.S.; Tatykhanova, G.S.; Kudaibergenov, S.E. In Situ Entrapment of Catalase within Macroporous Cryogel Matrix for Ethanol Oxidation: Flow-through Mode versus Batch Reactor. Catalysts 2023, 13, 1075.
Abstract
In this article, the biocatalytic oxidation of ethanol into acetaldehyde was studied using a catalase entrapped within monolithic polyampholyte cryogel, p(APTAC-co-AMPS), derived from an anionic monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS), and a cationic monomer, (3-acrylamidopropyl) trimethylammonium chloride (APTAC) as catalyst. Macroporous polyampholyte cryogels containing various amounts of catalase were synthesized in situ under cryo-polymerization conditions at a molar ratio of monomers [APTAC]:[AMPS] = 75:25 mol.% in the presence of 10 mol.% cross-linking agent, N,N-methylenebisacrylamide (MBAA). The conversion of ethanol into acetaldehyde in good-to-high yields was observed in flow-through and batch type reactors under optimal conditions: at T = 10-20 C, pH = 7.1, [C2H5OH]:[H2O2] = 50:50 vol.%. According to SEM image the pore sizes of p(AMPS-co-APTAC) cryogel vary from 15 to 55 μm. The catalytic activity of catalase entrapped within monolithic polyampholyte cryogel in the conversion of ethanol into acetaldehyde was evaluated through the determination of such kinetic parameters as Michaelis constant (Km), the maximum enzymatic rate (Vmax), activation energy (Ea), turnover number (TON) and turnover frequency (TOF). The catalase encapsulated within monolithic polyampholyte cryogel exhibits a high conversion of ethanol into acetaldehyde. The advantages and disadvantages of flow-through and batch type reactors were highlighted.
Keywords
macroporous cryogel; catalase; entrapment; oxidation of ethanol; hydrogen peroxide; molecular oxygen, air; flow-through reactor; batch type reactor
Subject
Chemistry and Materials Science, Chemical Engineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
