Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

An Ethyl Acetate Extract of Eryngium carlinae Inflorescences Attenuates Oxidative Stress and Inflammation in the Liver of Streptozotocin-Induced Diabetic Rats

Cristian M. Trejo-Hurtado
,
Cinthia I. Landa-Moreno
,
Jenaro Lemus-De la Cruz
,
Donovan J. Peña-Montes
,
Rocío Montoya-Pérez
ORCID logo ,
Rafael Salgado-Garciglia
,
Salvador Manzo-Avalos
,
Christian Cortés-Rojo
ORCID logo ,
Juan Luis Monribot-Villanueva
ORCID logo ,
José Antonio Guerrero-Analco
ORCID logo ,
Alfredo Saavedra-Molina
* ORCID logo
Version 1 : Received: 22 May 2023 / Approved: 24 May 2023 / Online: 24 May 2023 (03:06:23 CEST)

A peer-reviewed article of this Preprint also exists.

Trejo-Hurtado, C.M.; Landa-Moreno, C.I.; la Cruz, J.L.-D.; Peña-Montes, D.J.; Montoya-Pérez, R.; Salgado-Garciglia, R.; Manzo-Avalos, S.; Cortés-Rojo, C.; Monribot-Villanueva, J.L.; Guerrero-Analco, J.A.; Saavedra-Molina, A. An Ethyl Acetate Extract of Eryngium carlinae Inflorescences Attenuates Oxidative Stress and Inflammation in the Liver of Streptozotocin-Induced Diabetic Rats. Antioxidants 2023, 12, 1235. Trejo-Hurtado, C.M.; Landa-Moreno, C.I.; la Cruz, J.L.-D.; Peña-Montes, D.J.; Montoya-Pérez, R.; Salgado-Garciglia, R.; Manzo-Avalos, S.; Cortés-Rojo, C.; Monribot-Villanueva, J.L.; Guerrero-Analco, J.A.; Saavedra-Molina, A. An Ethyl Acetate Extract of Eryngium carlinae Inflorescences Attenuates Oxidative Stress and Inflammation in the Liver of Streptozotocin-Induced Diabetic Rats. Antioxidants 2023, 12, 1235.

Abstract

Secondary metabolites such as flavonoids are considered to be promising in the treatment of NAFLD, which is one of the complications of diabetes due to oxidative stress and inflammation. Some plants, such as Eryngium carlinae, have been investigated with regard to their medicinal properties in in vitro and in vivo assays, showing favorable results for the treatment of various diseases such as diabetes and obesity. The present study examined the antioxidant and anti-inflammatory effects of the phenolic compounds present in an ethyl acetate extract of the inflorescences of Eryngium carlinae on liver homogenates and mitochondria from STZ-induced diabetic rats. Phenolic compounds were identified and quantified by UHPLC-MS. In vitro assays were carried out to discover the antioxidant potential of the extract. Male Wistar rats were administered with a single intraperitoneal injection of STZ (45 mg/kg) and were given the ethyl acetate extract at a level of 30 mg/kg for 60 days. Phytochemical assays showed that the major constituents of the extract were flavonoids; in addition, the in vitro antioxidant activity was dose-dependent with IC50 = 57.97 mg/mL and IC50 = 30.90 mg/mL in the DPPH and FRAP assays, respectively. Moreover, the oral administration of the ethyl acetate extract improved the effects of NAFLD, decreasing serum and liver TG levels and oxidative stress markers and increasing the activity of the antioxidant enzymes. Likewise, it attenuated liver damage by decreasing the expression of NF-κB and iNOS, which lead to inflammation and liver damage. These results suggest that the phenolic compounds of the ethyl acetate extract of E. carlinae have antioxidant, anti-inflammatory, hypolipidemic, and hepatoprotective activity.

Keywords

antioxidants; anti-inflammatory activity; diabetes mellitus; Eryngium carlinae; phenolic compounds; rosmarinic acid

Subject

Medicine and Pharmacology, Complementary and Alternative Medicine

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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