Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs

Xiaoxiao Deng; Yuebo Zhang; Gang Song; Yawei Fu; Yue Chen; Hu Gao; Qian Wang; Zhao Jin; Yulong Yin; Kang Xu

doi:10.20944/preprints202305.1619.v1

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preprints.org > biology and life sciences > animal science, veterinary science and zoology > doi: 10.20944/preprints202305.1619.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs

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These authors contributed equally to the work.

Version 1 : Received: 23 May 2023 / Approved: 23 May 2023 / Online: 23 May 2023 (08:27:29 CEST)

How to cite: Deng, X.; Zhang, Y.; Song, G.; Fu, Y.; Chen, Y.; Gao, H.; Wang, Q.; Jin, Z.; Yin, Y.; Xu, K. Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs. Preprints 2023, 2023051619. https://doi.org/10.20944/preprints202305.1619.v1 Deng, X.; Zhang, Y.; Song, G.; Fu, Y.; Chen, Y.; Gao, H.; Wang, Q.; Jin, Z.; Yin, Y.; Xu, K. Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs. Preprints 2023, 2023051619. https://doi.org/10.20944/preprints202305.1619.v1

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MDPI and ACS Style

Deng, X.; Zhang, Y.; Song, G.; Fu, Y.; Chen, Y.; Gao, H.; Wang, Q.; Jin, Z.; Yin, Y.; Xu, K. Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs. Preprints 2023, 2023051619. https://doi.org/10.20944/preprints202305.1619.v1

APA Style

Deng, X., Zhang, Y., Song, G., Fu, Y., Chen, Y., Gao, H., Wang, Q., Jin, Z., Yin, Y., & Xu, K. (2023). Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs. Preprints. https://doi.org/10.20944/preprints202305.1619.v1

Chicago/Turabian Style

Deng, X., Yulong Yin and Kang Xu. 2023 "Integrative Analysis of Transcriptomic and Lipidomic Profiles Reveal Differential Mechanism of Subcutaneous Adipose Tissue among Ningxiang, Berkshire and Their Offspring Pigs" Preprints. https://doi.org/10.20944/preprints202305.1619.v1

Abstract

Adipose tissue composition contributes greatly to the quality and nutritional value of meat. Transcriptomic and lipidomic techniques were used to investigate the molecular mechanism of fat deposition difference among Ningxiang, Berkshire and F1 pigs. Transcriptomic analysis identified 680, 592 and 380 differentially expressed genes (DEGs) by comparing the groups of Ningxiang pigs vs Berkshire pigs, Berkshire pigs vs F1 pigs, Ningxiang pigs vs F1 pigs. Lipidomic analysis screened 423, 252, and 50 significantly changed lipids (SCLs) by comparing the groups of Ningxiang pigs vs Berkshire pigs, Berkshire pigs vs F1 pigs, Ningxiang pigs vs F1 pigs. Lycine, serine and threonine metabolism pathway, fatty acid biosynthesis and metabolism related pathways were siginificant enriched in the groups of Berkshire pigs vs Ningxiang pigs and Berkshire pigs vs F1 pigs. The DEGs (PHGDH, LOC110256000) and the SCLs (Phosphatidylserines) may have a great impact on lycine, serine and threonine metabolism pathway. Moreover, the DEGs (FASN, ACACA, CBR4, SCD, ELOV6, HACD, CYP3A46, CYP2B22, GPX1, GPX3) and the SCLs (palmitoleic acid, linoleic acid, arachidonic acid, icosadienoic acid) play important role in fatty acid biosynthesis and metabolism. Thus, the fat deposition difference among Ningxiang, Berkshire and F1 pigs may be caused by the difference of the expression pattern of key genes in multiple enrichment KEGG pathways. The research presented multiple lipids that were potentially available biological indicator and screened key genes that were potentially gene targets for molecular design breeding. The research also explored the molecular mechanisms of fat deposition difference among Ningxiang, Berkshire and F1 pigs and provides insight into the selection of backfat thickness and fat composition of adipose tissue for future breeding strategies.

Keywords

Pigs; subcutaneous adipose tissue; transcriptomic; lipidomic; KEGG pathways

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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