Lizana, R.R.; Camargos, R.S.; Silva, R.L.; Leme, B.B.; Sakomura, N.K. Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials. Animals2023, 13, 2675.
Lizana, R.R.; Camargos, R.S.; Silva, R.L.; Leme, B.B.; Sakomura, N.K. Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials. Animals 2023, 13, 2675.
Lizana, R.R.; Camargos, R.S.; Silva, R.L.; Leme, B.B.; Sakomura, N.K. Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials. Animals2023, 13, 2675.
Lizana, R.R.; Camargos, R.S.; Silva, R.L.; Leme, B.B.; Sakomura, N.K. Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials. Animals 2023, 13, 2675.
Abstract
Indirect calorimetry (IC) is a widely used method to study animal energy metabolism by measuring gas exchange. The accuracy of IC depends on detecting variations in signals reflecting the metabolic response, which can be challenging due to measurement noise and external factors. In this study, we propose a methodology to validate IC systems, including an easy-to-use spreadsheet for data computing, to verify accuracy and detect whole-system leaks. We conducted a re-covery test using a simulation of CO2 dynamics in MS Excel and injecting a known CO2 concentration into four respirometry chambers. The clearance rate of CO2 was observed and compared to the expected clearance rate from the simulation. The results from the recovery test in our system show that the proposed methodology is accurate and precise. The proposed methodology and recovery test can be used to standardize the validation of IC systems, providing accurate measurements of animal energy metabolism in different environmental conditions and energy utilization from feeds.
Keywords
farm anaimals,; farm animals; gas exchange; energy expenditure; metabolic rate; respirometric chambers
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
