Huang, S.-J.; Lan, J.-H.; Chiang, C.-C.; Lo, F.-Y.; Young, K.-C. Raman Spectrocopic and Surface-Enhanced Raman Spectrocopic Analyses of Normal Blood and Abnormal Blood. Preprints2023, 2023050053. https://doi.org/10.20944/preprints202305.0053.v1
APA Style
Huang, S. J., Lan, J. H., Chiang, C. C., Lo, F. Y., & Young, K. C. (2023). Raman Spectrocopic and Surface-Enhanced Raman Spectrocopic Analyses of Normal Blood and Abnormal Blood. Preprints. https://doi.org/10.20944/preprints202305.0053.v1
Chicago/Turabian Style
Huang, S., Fang-Yuh Lo and Kung-Chia Young. 2023 "Raman Spectrocopic and Surface-Enhanced Raman Spectrocopic Analyses of Normal Blood and Abnormal Blood" Preprints. https://doi.org/10.20944/preprints202305.0053.v1
Abstract
Blood testing is a crucial medical application. In this study, we applied Raman spectroscopy to test blood samples and obtained complete biological information, including the main components and compositions in these samples. Short-wavelength (532-nm green light) Raman scattering spectroscopy was conducted on samples of normal blood, abnormal blood in 3 types of preparation including whole blood, plasma, and serum, and the underlying information reflected by the biological characteristics detected in each sample type were analyzed. Raman spectroscopy results indicated that normal blood had high hemoglobin content, which suggests that hemoglobin is a major component of blood. Hemoglobin affects blood oxygen level. The characteristic peaks of hemoglobin were observed at 690, 989, 1015, 1182, 1233, 1315, and 1562–1649 cm−1. Analysis of plasma and serum samples indicated the presence of β-carotene, which exhibited characteristic peaks at 1013, 1172, and 1526 cm−1. In addition, surface-enhanced Raman spectroscopy was used to collect biological signals that are difficult to obtain in conventional Raman spectroscopy, including those of small molecules such as hormones, antibodies, and enzymes. Changes in biological information collected in this manner can be used as a basis for potentially diagnosing clinical diseases.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
