Xiao, C.; Guo, N.; Liang, Z.; Huang, Z.; Li, W.; Xie, M.; Zhao, F. Ultrafast Energy Transfer Dynamics in a Cyanobacterial Light-Harvesting Phycobilisome. Processes2023, 11, 1656.
Xiao, C.; Guo, N.; Liang, Z.; Huang, Z.; Li, W.; Xie, M.; Zhao, F. Ultrafast Energy Transfer Dynamics in a Cyanobacterial Light-Harvesting Phycobilisome. Processes 2023, 11, 1656.
Xiao, C.; Guo, N.; Liang, Z.; Huang, Z.; Li, W.; Xie, M.; Zhao, F. Ultrafast Energy Transfer Dynamics in a Cyanobacterial Light-Harvesting Phycobilisome. Processes2023, 11, 1656.
Xiao, C.; Guo, N.; Liang, Z.; Huang, Z.; Li, W.; Xie, M.; Zhao, F. Ultrafast Energy Transfer Dynamics in a Cyanobacterial Light-Harvesting Phycobilisome. Processes 2023, 11, 1656.
Abstract
The phycobilisomes (PBS) of cyanobacteria and red algae are the primary light-harvesting an-tennas, absorbing solar energy and transporting it to photosynthetic reaction centres with ex-traordinary efficiency and rate. The mechanism of energy transfer in PBS should be investigated in conjunction with biological structural information, as the functions of proteins result from structures. Here, we report the energy transfer study in PBS from a thermophilic cyanobacte-rium Thermosynechococcus vulcanus NIES 2134 (T. 2134), with the Cryo-EM model resolved at near-atomic-resolution recently. The time-resolved fluorescence spectroscopy of PBS with the sub-picosecond resolution was discovered at 77K. Deconvolution of the fluorescence decay curve was then used to reveal the energy transfer channels and the associated transfer rates. Expert for the fluorescence lifetimes of terminal emitters, four time-components, i.e., 9 ps, 13 ps, 23 ps, and 55 ps, were recognised in the energy transfer in PBS. The energy transfer dynamics in PBS were further analysed by combining the cryo-EM structure and the spectral property. The findings aid our understanding of the energy transfer mechanisms in PBS.
Keywords
light-harvesting antenna; phycobilisomes; time-resolved spectroscopy; energy transfer
Subject
Physical Sciences, Biophysics
Copyright:
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