PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection
Version 1
: Received: 23 April 2023 / Approved: 23 April 2023 / Online: 23 April 2023 (08:27:29 CEST)
How to cite:
Li, S.; Zhang, K.; Du, W.; Li, F. Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection. Preprints2023, 2023040794. https://doi.org/10.20944/preprints202304.0794.v1
Li, S.; Zhang, K.; Du, W.; Li, F. Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection. Preprints 2023, 2023040794. https://doi.org/10.20944/preprints202304.0794.v1
Li, S.; Zhang, K.; Du, W.; Li, F. Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection. Preprints2023, 2023040794. https://doi.org/10.20944/preprints202304.0794.v1
APA Style
Li, S., Zhang, K., Du, W., & Li, F. (2023). Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection. Preprints. https://doi.org/10.20944/preprints202304.0794.v1
Chicago/Turabian Style
Li, S., Wenran Du and Fuhua Li. 2023 "Two Independently Comparative Transcriptome Analyses of Hemocytes Provide New Insights into Understanding the Disease-Resistant Characteristics of Shrimp against Vibrio Infection" Preprints. https://doi.org/10.20944/preprints202304.0794.v1
Abstract
Vibrio parahaemolyticus carrying a plasmid encoding toxins PirA and PirB is one of the causative agents leading to the severe disease of acute hepatopancreatic necrosis disease (AHPND) in shrimp aquaculture. However, there is a lack of deep understanding of the host resistant characteristics against V. parahaemolyticus infection. Here, we performed comparative transcriptome analysis on the expression profiles at the background level of hemocytes from shrimp with different resistant abilities against V. parahaemolyticus infection in two independent populations. Principal component analysis and sample clustering results showed that samples from the same population had a closer relationship than that from shrimp with similar disease-resistant abilities. Differentially expressed genes (DEGs) analysis revealed that the number of DEGs between two populations was much more than that between V. parahaemolyticus¬-resistant and susceptible shrimp. A total of 31 DEGs and 5 DEGs were identified from the comparison between V. parahaemolyticus¬-resistant and susceptible shrimp from population 1 and 2, respectively. There was no DEGs overlap between two comparisons. DEGs from population 1 were mainly cytoskeleton related genes, metabolic related genes, and immune related genes. DEGs from population 2 included tubulin alpha-3, dihydropyrimidinase-like gene, diacylglycerol kinase 1, prohibitin and iroquois-class homeodomain protein IRX-2-like. The data suggest that shrimp from different populations might adopt distinct disease-resistant mechanisms, which provided new insights into understanding disease-resistant mechanisms at population level against pathogen infection. A new strategy for screening disease resistant genes and disease resistant breeding was proposed based on the present study.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
