Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In situ identification of both IL-4 and IL-10 Cytokine-Receptor Interactions during tissue regeneration

Krisztina Nikovics
* ORCID logo ,
Anne-Laure Favier
,
Mathilde Rocher
,
Céline Mayinga
,
Johanna Gomez
,
Frédérique Dufour-Gaume
,
Diane Riccobono
Version 1 : Received: 21 April 2023 / Approved: 23 April 2023 / Online: 23 April 2023 (03:25:00 CEST)

A peer-reviewed article of this Preprint also exists.

Nikovics, K.; Favier, A.-L.; Rocher, M.; Mayinga, C.; Gomez, J.; Dufour-Gaume, F.; Riccobono, D. In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration. Cells 2023, 12, 1522. Nikovics, K.; Favier, A.-L.; Rocher, M.; Mayinga, C.; Gomez, J.; Dufour-Gaume, F.; Riccobono, D. In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration. Cells 2023, 12, 1522.

Abstract

Cytokines secreted by individual immune cells regulate tissue regeneration and allow communication between various cell types. Cytokines bind to cognate receptors and trigger the healing process. Determining the orchestration of cytokine interactions with their receptors on their cellular targets is essential to fully understand the process of inflammation and tissue regeneration. To this end, we have investigated the interactions of Interleukin-4 cytokine (IL-4)/Interleukin-4 cytokine receptor (IL-4R) and Interleukin-10 cytokine (IL-10)/Interleukin-10 cytokine receptor (IL-10R) using in situ Proximity Ligation Assays in a regenerative model of skin, muscle and lung tissues in the mini-pig. The pattern of protein-protein interactions was distinct for the two cytokines. IL-4 bound predominantly to receptors on macrophages and endothelial cells around the blood vessels while the target cells of IL-10 were mainly receptors on muscle cells. Our results show that in situ studies of cytokine-receptor interactions can provide unravel the fine details of the mechanism of action of cytokines.

Keywords

IL-4; IL-10; receptor; interaction; regeneration; Proximity ligation assay

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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