Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Are Antarctic Fish Adapted to Face Global Changes? A Study on Antioxidant Enzymes Gene Expression in Trematomus newnesi from Ross Sea, Experimentally Exposed to PFOA

Sara Pacchini
ORCID logo ,
Elisabetta Piva
,
Sophia Schumann
ORCID logo ,
Paola Irato
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Daniela Pellegrino
ORCID logo ,
Gianfranco Santovito
* ORCID logo
Version 1 : Received: 31 December 2022 / Approved: 5 January 2023 / Online: 5 January 2023 (04:50:42 CET)

A peer-reviewed article of this Preprint also exists.

Pacchini, S.; Piva, E.; Schumann, S.; Irato, P.; Pellegrino, D.; Santovito, G. An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid. Antioxidants 2023, 12, 352. Pacchini, S.; Piva, E.; Schumann, S.; Irato, P.; Pellegrino, D.; Santovito, G. An Experimental Study on Antioxidant Enzyme Gene Expression in Trematomus newnesi (Boulenger, 1902) Experimentally Exposed to Perfluoro-Octanoic Acid. Antioxidants 2023, 12, 352.

Abstract

Antarctica is the continent with the lowest local human impact, yet it is still vulnerable to contaminants coming from external sources. Emerging pollutants, like PFAS, pose an increasing threat to this environment and therefore require more in-depth investigations to understand their environmental fate and biological impacts. The present study, part of the AntaGPS project, focuses on expression analysis at the transcriptional level of genes coding for 4 antioxidant enzymes (sod1, sod2, gpx1, gpx4) in different organs of an Antarctic fish species, Trematomus newnesi. The kidney showed a higher level of expression than the liver of wildlife specimens. The mRNA levels were also assessed in fish exposed to 1.5 μg/L of PFOA for 10 days. In the liver, the treatment induced an increase in gene expression for all the considered enzymes, while in the kidney it induced a general decrease. The obtained results constitute a starting point for using the expression of antioxidant enzymes as biomarkers, both of oxidative stress and exposure to PFAS, in future biomonitoring campaigns in the Antarctic marine environment.

Keywords

Antarctic fish; antioxidant enzymes; oxidative stress; gene expression; PFAS

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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