Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multiplex Specific IgE Profiling in Neonatal Stool of Preterms Predicts IgE-mediated Disease

Youssouf Sereme
ORCID logo ,
Moïse Michel
ORCID logo ,
Soraya Mezouar
ORCID logo ,
Nicolas Orain
,
Renaud Cezar
,
Tu Anh Tran
,
Pierre Corbeau
,
Anne Filleron
,
Joana Vitte
* ORCID logo
Version 1 : Received: 17 December 2022 / Approved: 20 December 2022 / Online: 20 December 2022 (10:15:32 CET)

How to cite: Sereme, Y.; Michel, M.; Mezouar, S.; Orain, N.; Cezar, R.; Tran, T.A.; Corbeau, P.; Filleron, A.; Vitte, J. Multiplex Specific IgE Profiling in Neonatal Stool of Preterms Predicts IgE-mediated Disease. Preprints 2022, 2022120365. https://doi.org/10.20944/preprints202212.0365.v1 Sereme, Y.; Michel, M.; Mezouar, S.; Orain, N.; Cezar, R.; Tran, T.A.; Corbeau, P.; Filleron, A.; Vitte, J. Multiplex Specific IgE Profiling in Neonatal Stool of Preterms Predicts IgE-mediated Disease. Preprints 2022, 2022120365. https://doi.org/10.20944/preprints202212.0365.v1

Abstract

Background: Little is known about the natural history of immunoglobulin (Ig) E-mediated diseases in preterm infants, further hampered by the lack of noninvasive investigations. We aimed at developing a non-invasive tool for the study of IgE and eosinophil-derived neurotoxin (EDN) in neonatal fecal samples and evaluating its predictive value for the development of IgE-mediated diseases (cow’s milk allergy, asthma, or atopic dermatitis) during the first year of life. Methods: We developed a stool extraction protocol, followed by freeze-drying and solubilization. sIgE responses were investigated in neonatal fecal samples from 21 preterm infants with a 300-allergen multiplex containing whole and molecular allergens and confirmed by capillary Western blot with nano-immunoassay. The local eosinophilic component was investigated by measuring the concentration of EDN. Results: The multiplexed allergen assay detected sIgE in all samples. Confirmation was obtained with Western blot. Frequency and levels of sIgE in neonatal fecal samples differed between infants who developed IgE-mediated diseases and controls. Neonatal fecal sIgE directed to milk proteins predicted later development of cow’s milk allergy (specificity 88%, sensitivity 78%). Allergen specificity of neonatal fecal sIgE was associated with later development of cow’s milk allergy and asthma. Neonatal fecal EDN levels predicted the development of IgE-mediated diseases (sensitivity 100%, specificity 75%). Conclusion: Non-invasive investigation of neonatal fecal sIgE is a promising tool for the prediction of subsequent development of IgE-mediated diseases.

Keywords

Fecal IgE; preterm birth; neonate; asthma; atopic dermatitis; cow’s milk allergy; Western Blot

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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