PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Changes in Nitrate Concentration, Nitrate Reductase Activity, and Accumulation of Nutrients in Spinach (Spinacia Oleracea L.) in Response to Streptomyces Sp. Inoculation
Version 1
: Received: 31 October 2022 / Approved: 2 November 2022 / Online: 2 November 2022 (02:59:42 CET)
How to cite:
Saraylou, M.; Nadian, H. A.; Enayatizamir, N.; Rangzan, N.; Senn, S. Changes in Nitrate Concentration, Nitrate Reductase Activity, and Accumulation of Nutrients in Spinach (Spinacia Oleracea L.) in Response to Streptomyces Sp. Inoculation. Preprints2022, 2022110036. https://doi.org/10.20944/preprints202211.0036.v1
Saraylou, M.; Nadian, H. A.; Enayatizamir, N.; Rangzan, N.; Senn, S. Changes in Nitrate Concentration, Nitrate Reductase Activity, and Accumulation of Nutrients in Spinach (Spinacia Oleracea L.) in Response to Streptomyces Sp. Inoculation. Preprints 2022, 2022110036. https://doi.org/10.20944/preprints202211.0036.v1
Saraylou, M.; Nadian, H. A.; Enayatizamir, N.; Rangzan, N.; Senn, S. Changes in Nitrate Concentration, Nitrate Reductase Activity, and Accumulation of Nutrients in Spinach (Spinacia Oleracea L.) in Response to Streptomyces Sp. Inoculation. Preprints2022, 2022110036. https://doi.org/10.20944/preprints202211.0036.v1
APA Style
Saraylou, M., Nadian, H. A., Enayatizamir, N., Rangzan, N., & Senn, S. (2022). Changes in Nitrate Concentration, Nitrate Reductase Activity, and Accumulation of Nutrients in Spinach (<em>Spinacia Oleracea</em> L.) in Response to <em>Streptomyces</em> Sp. Inoculation. Preprints. https://doi.org/10.20944/preprints202211.0036.v1
Chicago/Turabian Style
Saraylou, M., Nafiseh Rangzan and Savanah Senn. 2022 "Changes in Nitrate Concentration, Nitrate Reductase Activity, and Accumulation of Nutrients in Spinach (<em>Spinacia Oleracea</em> L.) in Response to <em>Streptomyces</em> Sp. Inoculation" Preprints. https://doi.org/10.20944/preprints202211.0036.v1
Abstract
A replicated outdoor pot experiment was conducted in order to investigate the effect of different phytohormone and siderophore producing, and P-solubilizing bacterial species on spinach nu-trient uptake, nitrate concentration and nitrate reductase activity. The mentioned parameters were determined in spinach leaves (Spinacia oleracea L.), non-inoculated and inoculated with four plant growth–promoting actinobacteria (Streptomyces griseus (S1), Streptomyces albogriseolus (S2), Strep-tomyces aurantiacus (S3) and Streptomyces kanamyceticus (S4) under the influence of two sources of nitrogen fertilizers including potassium nitrate and urea (100 and 200 ppm). Inoculation with the strains increased spinach shoot fresh weight by 16%–43% over the control. Bacterial inoculation gave leaf chlorophyll increases of 15%–40%. Inoculation increased plant height by 2.2%–24.6% in spinach. A close reverse relationship between nitrate concentration and enzyme activity (r2= 0.87) was demonstrated. The measured parameter responses were variable and dependent on the in-oculant strain, with highest enzyme activity and lowest nitrate concentration exhibited in S2 (S. albogriseolus) inoculation. The source and application dose of nitrogen fertilizer had varied impact on measured parameter. The maximum phosphorous and iron concentration were measured by soil inoculation with S4 and applying 200 ppm nitrate potassium nitrogen fertilizer. Application of 200 ppm KNO3 nitrogen fertilizer with different Streptomyces strains showed the capability of S2 in decreasing nitrate content while protein content increased. In particular, the strains S2 and S4 have great potential in being formulated and used as biofertilizers.
Keywords
Streptomyces; vegetable production; biofertilizer
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
