Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Strategies for Generating Human Pluripotent Stem Cell- Derived-Organoid Culture for Disease Modelling, Drug Screening, and Regenerative Therapy

Zakiya Gania
ORCID logo ,
Syarifah Tiara Noorintan
ORCID logo ,
Ni Putu Diah Pradnya Septiari
,
Dhea Sandra Fitriany
,
Fuad Gandhi Torizal
* ORCID logo
Version 1 : Received: 22 June 2022 / Approved: 24 June 2022 / Online: 24 June 2022 (08:11:15 CEST)

A peer-reviewed article of this Preprint also exists.

Gania, Z.; Noorintan, S.T.; Septiari, N.P.D.P.; Fitriany, D.S.; Torizal, F.G. Strategies for Generating Human Pluripotent Stem Cell-Derived-Organoid Culture for Disease Modeling, Drug Screening, and Regenerative Therapy. Future Pharmacol. 2022, 2, 360-376. Gania, Z.; Noorintan, S.T.; Septiari, N.P.D.P.; Fitriany, D.S.; Torizal, F.G. Strategies for Generating Human Pluripotent Stem Cell-Derived-Organoid Culture for Disease Modeling, Drug Screening, and Regenerative Therapy. Future Pharmacol. 2022, 2, 360-376.

Abstract

Human pluripotent stem cells (hPSCs) have become a powerful tool to generate various kinds of cell types comprising the human body. Recently, organoid technology emerged as a platform to build a physiologically relevant tissue-like structure from the PSCs, which provides a more relevant three-dimensional microenvironment to the actual human body than the conventional monolayer culture system for transplantation, disease modeling, and drug development. Although it holds so many advantages, the organoid culture system still has various problems related to culture methods, which became a challenge to get similar physiological properties to their original tissue counterparts. Here, we discuss the current development of organoid culture methods, including the problem that may arise from the currently available culture systems as well as the possible approach to overcoming the current limitation and improving their optimum utilization for translational application purposes.

Keywords

hPSCs derived-organoids; Culture strategy; Disease modeling; Drug screening; Regenerative therapy

Subject

Medicine and Pharmacology, Medicine and Pharmacology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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