Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Version 1 : Received: 4 May 2022 / Approved: 6 May 2022 / Online: 6 May 2022 (13:58:59 CEST)

A peer-reviewed article of this Preprint also exists.

Fanalli, S.L.; da Silva, B.P.M.; Gomes, J.D.; de Almeida, V.V.; Freitas, F.A.O.; Moreira, G.C.M.; Silva-Vignato, B.; Afonso, J.; Reecy, J.; Koltes, J.; Koltes, D.; de Almeida Regitano, L.C.; Garrick, D.J.; de Carvalho Balieiro, J.C.; Meira, A.N.; Freitas, L.; Coutinho, L.L.; Fukumasu, H.; Mourão, G.B.; de Alencar, S.M.; Luchiari Filho, A.; Cesar, A.S.M. Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs. Animals 2022, 12, 1632. Fanalli, S.L.; da Silva, B.P.M.; Gomes, J.D.; de Almeida, V.V.; Freitas, F.A.O.; Moreira, G.C.M.; Silva-Vignato, B.; Afonso, J.; Reecy, J.; Koltes, J.; Koltes, D.; de Almeida Regitano, L.C.; Garrick, D.J.; de Carvalho Balieiro, J.C.; Meira, A.N.; Freitas, L.; Coutinho, L.L.; Fukumasu, H.; Mourão, G.B.; de Alencar, S.M.; Luchiari Filho, A.; Cesar, A.S.M. Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs. Animals 2022, 12, 1632.

Journal reference: Animals 2022, 12, 1632
DOI: 10.3390/ani12131632

Abstract

The aim of this study was to identify the differentially expressed genes (DEG) from the skeletal muscle and liver samples of animal model for metabolic diseases in human. To perform the study, the fatty acid (FA) profile and RNA sequencing (RNA-Seq) data of 35 samples of liver tissue (SOY1.5, n=17 and SOY3.0, n=18) and 36 samples of skeletal muscle (SOY1.5, n=18 and SOY3.0, n=18) of Large White pigs were analyzed. The FA profile of the tissues was modified by the diet, mainly those related to monounsaturated (MUFA) and polyunsaturated (PUFA) FA. The skeletal muscle transcriptome analysis revealed 45 DEG (FDR 10%), and the functional enrichment analysis identified network maps related to inflammation, immune process, and pathways associated with the oxidative stress, type 2 diabetes and metabolic dysfunction. For the liver tissue, the transcriptome profile analysis revealed 281 DEG, which participate in network maps related to neurodegenerative diseases. With this nutrigenomics study, we verified that different levels of soybean oil in the pig diet, an animal model for metabolic diseases in humans, affected the transcriptome profile of skeletal muscle and liver tissue. These findings may help to better understand the biological mechanisms that can be modulated by the diet.

Keywords

fatty acid; RNAseq; transcriptome; immune response; Huntington's disease; metabolism; hepatic tissue; Longissimus lomborum; biomedical model

Subject

LIFE SCIENCES, Molecular Biology

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