Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Skeletal Muscle Cells Derived from Induced Pluripotent Stem Cells: A Platform for Limb Girdle Muscular Dystrophies

Celine Bruge
,
Marine Geoffroy
ORCID logo ,
Manon Benabides
,
Emilie Pellier
,
Jamila Dhiab
,
Evelyne Gicquel
,
Lucile Hoch
,
Isabelle Richard
ORCID logo ,
Xavier Nissan
* ORCID logo
Version 1 : Received: 28 April 2022 / Approved: 5 May 2022 / Online: 5 May 2022 (03:44:03 CEST)

A peer-reviewed article of this Preprint also exists.

Bruge, C.; Geoffroy, M.; Benabides, M.; Pellier, E.; Gicquel, E.; Dhiab, J.; Hoch, L.; Richard, I.; Nissan, X. Skeletal Muscle Cells Derived from Induced Pluripotent Stem Cells: A Platform for Limb Girdle Muscular Dystrophies. Biomedicines 2022, 10, 1428. Bruge, C.; Geoffroy, M.; Benabides, M.; Pellier, E.; Gicquel, E.; Dhiab, J.; Hoch, L.; Richard, I.; Nissan, X. Skeletal Muscle Cells Derived from Induced Pluripotent Stem Cells: A Platform for Limb Girdle Muscular Dystrophies. Biomedicines 2022, 10, 1428.

DOI: 10.3390/biomedicines10061428

Abstract

Limb girdle muscular dystrophies (LGMD), caused by mutations in 29 different genes, are the fourth most prevalent group of genetic muscle diseases, leading to progressive weakness and atrophy of the skeletal muscles. Although the link between LGMD and their genetic origins has been determined, LGMD still represent an unmet medical need. In this paper, we describe a platform for modeling LGMD based on the use of human induced pluripotent stem cells (hiPSC). Thanks to the self-renewing and pluripotency properties of hiPSC, this platform provides an alternative and renewable source of skeletal muscle cells (skMC) to primary, immortalized or overexpressing cells. We report that skMC derived from hiPSC express the majority of the genes and proteins causing LGMD. As a proof of concept, we demonstrate the importance of this cellular model for studying LGMDR9 by evaluating disease-specific phenotypes in skMC derived from hiPSC obtained from four patients.

Keywords

limb girdle muscular dystrophies; induced pluripotent stem cells; skeletal muscle cells; pathological modeling

Subject

BIOLOGY, Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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