Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis

Version 1 : Received: 14 January 2022 / Approved: 20 January 2022 / Online: 20 January 2022 (13:41:39 CET)

How to cite: Alqalshy, E.; Ibrahim, A.M.; Abdel-Hafiz, A.A.; AbdEl-Rahman, K.A.; Alazzazi, M.; Omar, M.; Abdel-Wahab, A.S. Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis. Preprints 2022, 2022010307 (doi: 10.20944/preprints202201.0307.v1). Alqalshy, E.; Ibrahim, A.M.; Abdel-Hafiz, A.A.; AbdEl-Rahman, K.A.; Alazzazi, M.; Omar, M.; Abdel-Wahab, A.S. Effect of Docosahexaenoic Acid as a Chemopreventive Agent on Experimentally Induced Hamster Buccal Pouch Carcinogenesis. Preprints 2022, 2022010307 (doi: 10.20944/preprints202201.0307.v1).

Abstract

The purpose of the current study was directed to investigate the effectiveness of docosahexaenoic acid (DHA) as a chemopreventive agent on experimentally induced hamster buccal pouch (HBP) carcinogenesis; Material and methods: 40 Syrian male hamsters, five weeks old, were divided into 4 groups of 10 animals in each as follows, GI: Topical application of liquid paraffin alone (thrice a week for 14 weeks), GII: Topical application of 7, 12 dimethyl benz[a]anthracene (DMBA) alone (0.5 % in liquid paraffin, thrice a week for 14 weeks), GIII: Topical application of DMBA (0.5 % in liquid paraffin, thrice a week for 14 weeks) + Oral administration of DHA (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks on alternative days of DMBA application), GIV : Oral administration of DHA alone (125 mg/kg b.w. in 1 ml distilled water by oral gavage, thrice a week for 14 weeks); Results: Gross observations and histopathological findings revealed a-GI: normal stratified squamous epithelium b- GII: well and moderately differentiated squamous cell carcinoma (SCC) c-: GIII: showed variable results ranges from hyperkeratosis, hyperkeratosis and focal hyperplasia, mild dysplasia, and well differentiated SCC with superficial invasion of tumor cells not extended to deeper areas d: GIV: normal similar to GI. Immunohistochemical results revealed that oral DHA treatment to DMBA treated hamsters restored the normal expression of bcl-2; Conclusion: DHA has the potential to be a dietary chemopreventive agent due to its capacity to improve carcinogen detoxification and to block/suppress the initiation and promotion stages of experimentally produced HBP carcinogenesis.

Keywords

Docosahexaenoic acid; Chemoprevention; Bcl-2 family; Experimental Study; Hamster Buccal Pouch Carcinogenesis.

Subject

MEDICINE & PHARMACOLOGY, Dentistry

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