Preprint Article Version 3 Preserved in Portico This version is not peer-reviewed

Identification of Novel Multi-Omics Expression Landscapes and Meta-Analysis of Landscape-Based Competitive Endogenous RNA Networks in ALDH+ Lung Adenocarcinoma Stem Cells

Version 1 : Received: 17 January 2022 / Approved: 20 January 2022 / Online: 20 January 2022 (07:49:16 CET)
Version 2 : Received: 16 May 2022 / Approved: 16 May 2022 / Online: 16 May 2022 (10:56:02 CEST)
Version 3 : Received: 4 August 2022 / Approved: 4 August 2022 / Online: 4 August 2022 (16:29:49 CEST)

How to cite: Yang, W.; Liang, Y.; Zheng, Y.Y.; Luo, H.T.; Yang, X.F.; Li, F.R. Identification of Novel Multi-Omics Expression Landscapes and Meta-Analysis of Landscape-Based Competitive Endogenous RNA Networks in ALDH+ Lung Adenocarcinoma Stem Cells. Preprints 2022, 2022010286 (doi: 10.20944/preprints202201.0286.v3). Yang, W.; Liang, Y.; Zheng, Y.Y.; Luo, H.T.; Yang, X.F.; Li, F.R. Identification of Novel Multi-Omics Expression Landscapes and Meta-Analysis of Landscape-Based Competitive Endogenous RNA Networks in ALDH+ Lung Adenocarcinoma Stem Cells. Preprints 2022, 2022010286 (doi: 10.20944/preprints202201.0286.v3).

Abstract

ALDH+ H1975 lung adenocarcinoma stem cells (LSCs) are a rare cell population identified in lung adenocarcinoma (LUAD). LSCs can self-renew, drive tumor initiation, growth, metastasis, and recurrence, and are also the predominant cause of poor prognosis due to their intrinsic resistance to drugs and chemotherapy. Consequently, LSCs are a promising target for LUAD therapy. Noncoding RNAs (ncRNAs), including microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), exert many significant regulatory functions in the pathogenesis of human cancers, showing the necessity for a comprehensive understanding of the mechanisms that underlie lung carcinogenesis. Nonetheless, research on many known transcripts and messenger RNAs (mRNAs) has already generated new information. Unknown biomarkers in ncRNAs and systematic and comprehensive interrelation with unknown ncRNAs and mRNAs may provide further insights into the biology of LUAD. Herein, a set of novel ncRNAs that include miRNAs, lncRNAs, circRNAs, were identified and differentially expressed patterns of ncRNAs and mRNAs in LSCs and ALDH- H1975 LUAD tumor cells (LTCs) were obtained using stringent bioinformatics pipelines. Through a meta-analysis of the identified landscapes, novel competitive endogenous RNA (ceRNA) networks were constructed to reveal the potential molecular mechanisms that regulate the hallmarks of LSCs and LTCs. This study presents a summary of novel ncRNAs and the fundamental roles of differentially expressed ncRNAs implicated in the activity of LSCs and LTCs. In addition, the study also provides a comprehensive resource for the future identification of diagnostic, therapeutic, and prognostic biomarkers in LUAD.

Keywords

novel ncRNAs; DE landscape; LSCs and LTCs; LncRNA; MiRNA; CircRNA; ceRNA networks; molecular mechanism

Subject

MEDICINE & PHARMACOLOGY, Oncology & Oncogenics

Comments (1)

Comment 1
Received: 4 August 2022
Commenter: Wei Yang
Commenter's Conflict of Interests: Author
Comment: 1 Following the suggestion of the reviewer, we have added details of the hub molecule selection process in Materials and Methods Section 2.7
2 The contents of Results sections 3.3 and 3.4 that discuss data have been eliminated in Lines 295-297, 364-366, 369-377, 379, and 390-393 and have been moved into the Discussion section.
3 We have carefully proofread the revised manuscript and amended the small spelling errors.
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