Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Estrogen Receptors, ERK 1/2 Phosphorylation and Reactive Oxidizing Species in Red Blood Cells From Patients With Rheumatoid Arthritis

Version 1 : Received: 21 December 2021 / Approved: 22 December 2021 / Online: 22 December 2021 (12:25:17 CET)

How to cite: Di Franco, M.; Gambardella, L.; Cittadini, C.; Favretti, M.; Gioia, C.; Vona, R.; Straface, E.; Pietraforte, D. Estrogen Receptors, ERK 1/2 Phosphorylation and Reactive Oxidizing Species in Red Blood Cells From Patients With Rheumatoid Arthritis. Preprints 2021, 2021120362 (doi: 10.20944/preprints202112.0362.v1). Di Franco, M.; Gambardella, L.; Cittadini, C.; Favretti, M.; Gioia, C.; Vona, R.; Straface, E.; Pietraforte, D. Estrogen Receptors, ERK 1/2 Phosphorylation and Reactive Oxidizing Species in Red Blood Cells From Patients With Rheumatoid Arthritis. Preprints 2021, 2021120362 (doi: 10.20944/preprints202112.0362.v1).

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease associated with a significantly increased risk of cardiovascular mortality, mainly attributed to accelerated atherosclerosis. Methods: Thirty-two women (aged more than 18 years) with RA, and 25 age-matched healthy women were included in this study. Biomarkers of inflammation, red blood cells (RBCs) redox balance, estrogen receptor alpha (ER-α) expression as well as ERK 1/2 phosphorylation content were evaluated in RA patients at baseline and six months after treatment with disease modifying anti‐rheumatic drugs (DMARDs). Results: For the first times we demonstrated that in RA patients: i) disease activity score (DAS-28) positively correlated with RBC ER-α expression, and negatively with total antioxidant capacity of plasma; ii) RBC ER-α expression positively correlated with systemic inflammatory biomarkers and oxidative stress parameters as well as ERK 1/2 phosphorylation; and iii) DMARDs treatments improved the clinical condition measured by DAS-28 score decrease, although the RBCs appeared to be more prone to pro-oxidant status associated to the expression of survival molecules. Conclusion: Our data strongly suggest that RBCs could also participate in vascular homeostasis through fine modulation of an intracellular signal linked to the ER-α.

Keywords

rheumatoid arthritis; inflammation, oxidative stress; red blood cells; estrogen receptors.

Subject

MEDICINE & PHARMACOLOGY, Other

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