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Current and Future Methodology for Quantitation and Site-specific Mapping the Location of DNA Adducts
Version 1
: Received: 16 December 2021 / Approved: 20 December 2021 / Online: 20 December 2021 (09:53:53 CET)
A peer-reviewed article of this Preprint also exists.
Boysen, G.; Nookaew, I. Current and Future Methodology for Quantitation and Site-Specific Mapping the Location of DNA Adducts. Toxics 2022, 10, 45. Boysen, G.; Nookaew, I. Current and Future Methodology for Quantitation and Site-Specific Mapping the Location of DNA Adducts. Toxics 2022, 10, 45.
Abstract
Abstract: Formation of DNA adducts is a key event for a genotoxic mode of action and its formation is often use as surrogate for mutation and cancer. Interest in DNA adducts are twofold, first, to demonstrate exposure, and second, to link DNA adduct location to subsequent mutations or altered gene regulation. High chemically specific mass spectrometry methods have been established for DNA adduct quantitation and elegant bio-analytic methods utilizing enzymes, various chemistries, and molecular biology methods to visualize the location of DNA adducts. Traditionally, these highly specific methods cannot be combined, and the results are incomparable. Initially developed for single-molecule DNA sequencing, nanopore-type technologies are expected to enable simultaneous quantitation and location of DNA adducts across the genome. We will briefly summarize the current methodologies for state-of-the-art quantitation of DNA adduct levels and mapping of DNA adducts and describe novel single-molecule DNA sequencing technology that is expected to achieve both measures simultaneously. Emerging technologies are expected to soon provide a comprehensive picture of the exposome and identify gene regions susceptible to DNA adduct formation.
Keywords
DNA adducts; nanopore; Oxford Nanopore Technology; mass spectrometry; adductomics; exposome
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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