Version 1
: Received: 1 December 2021 / Approved: 2 December 2021 / Online: 2 December 2021 (10:08:35 CET)
Version 2
: Received: 6 December 2021 / Approved: 7 December 2021 / Online: 7 December 2021 (12:35:12 CET)
How to cite:
Cruz-Ramos, J.A.; López-Armas, G.D.C.; Díaz-Barba, E.I.; Navarro-Meza, M.; Macías-Islas, M.Á.; Saldaña-Cruz, A.M.; Zepeda-Moreno, A.; Pérez-Gómez, H.R.; Martínez-Hernández, A.; Ramos-Márquez, M.E. Telomere Shortening linked to Disability and Mitochondrial DNA Copy Number in Patients with Relapsing-Remitting Multiple Sclerosis. Preprints2021, 2021120026. https://doi.org/10.20944/preprints202112.0026.v2
Cruz-Ramos, J.A.; López-Armas, G.D.C.; Díaz-Barba, E.I.; Navarro-Meza, M.; Macías-Islas, M.Á.; Saldaña-Cruz, A.M.; Zepeda-Moreno, A.; Pérez-Gómez, H.R.; Martínez-Hernández, A.; Ramos-Márquez, M.E. Telomere Shortening linked to Disability and Mitochondrial DNA Copy Number in Patients with Relapsing-Remitting Multiple Sclerosis. Preprints 2021, 2021120026. https://doi.org/10.20944/preprints202112.0026.v2
Cruz-Ramos, J.A.; López-Armas, G.D.C.; Díaz-Barba, E.I.; Navarro-Meza, M.; Macías-Islas, M.Á.; Saldaña-Cruz, A.M.; Zepeda-Moreno, A.; Pérez-Gómez, H.R.; Martínez-Hernández, A.; Ramos-Márquez, M.E. Telomere Shortening linked to Disability and Mitochondrial DNA Copy Number in Patients with Relapsing-Remitting Multiple Sclerosis. Preprints2021, 2021120026. https://doi.org/10.20944/preprints202112.0026.v2
APA Style
Cruz-Ramos, J.A., López-Armas, G.D.C., Díaz-Barba, E.I., Navarro-Meza, M., Macías-Islas, M.Á., Saldaña-Cruz, A.M., Zepeda-Moreno, A., Pérez-Gómez, H.R., Martínez-Hernández, A., & Ramos-Márquez, M.E. (2021). Telomere Shortening linked to Disability and Mitochondrial DNA Copy Number in Patients with Relapsing-Remitting Multiple Sclerosis. Preprints. https://doi.org/10.20944/preprints202112.0026.v2
Chicago/Turabian Style
Cruz-Ramos, J.A., Alejandra Martínez-Hernández and Martha Eloísa Ramos-Márquez. 2021 "Telomere Shortening linked to Disability and Mitochondrial DNA Copy Number in Patients with Relapsing-Remitting Multiple Sclerosis" Preprints. https://doi.org/10.20944/preprints202112.0026.v2
Abstract
Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease that affects the nervous system. Peripheral blood leukocyte telomere length (LTL) and mitochondrial DNA copy number (mtDNA-CN) are potential biomarkers of disability and neurological damage. The present work evaluated LTL and mtDNA-CN in 75 relapsing-remittent MS (RRMS) patients 50 of whom had an Expanded Disability Status Scale (EDSS) 0 to 3 (mild-moderate disability), and 25 had an EDSS of 3.5 to 7 (severe disability). Absolute LTL and absolute mtDNA-CN were measured via real-time polymerase chain reaction (qPCR). The LTL and mtDNA-CN were significantly lower in RRMS severe disability than in RRMS mild-moderate disability (3.924 ± 0.124 vs 2.854 ± 0.092, p<00001; 75.14 ± 1.77 vs 68.06 ± 1.608, p<0.00001, respectively). The LTL and mtDNA-CN showed a linear correlation in RRMS with mild-moderate disability (r=0.2986, p=0.0351). In addition, in a binary logistic regression model the LTL can predict severe disability (AUC=0.697, p=0.0031, cutoff ≤ 3.0875 Kb, sensitivity= 73.1%, specificity=62.5%), the prediction is improved by including age to the model (AUC=0.765, <0.0001, sensitivity=78.26%, specificity=81.25%). Aging is closely linked to the development of disability in RRMS and can be evaluated through LTL and mtDNA-CN absolute quantification.
Medicine and Pharmacology, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: Jose Alfonso Cruz-Ramos
Commenter's Conflict of Interests: Author
Some legends of the figures were modified.
The methods section was repositioned.