Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Mesenchymal Stem Cells Remodeling of Adsorbed Type I Collagen – Effect of Collagen Oxidation

Regina Komsa Penkova
* ORCID logo ,
Galya Stavreva
ORCID logo ,
Stanimir Kyurkchiev
,
Kalina Belemezova
ORCID logo ,
Svetla Todinova
ORCID logo ,
George Altankov
*
Version 1 : Received: 16 October 2021 / Approved: 19 October 2021 / Online: 19 October 2021 (12:19:16 CEST)

A peer-reviewed article of this Preprint also exists.

Komsa-Penkova, R.; Stavreva, G.; Belemezova, K.; Kyurkchiev, S.; Todinova, S.; Altankov, G. Mesenchymal Stem-Cell Remodeling of Adsorbed Type-I Collagen—The Effect of Collagen Oxidation. International Journal of Molecular Sciences 2022, 23, 3058, doi:10.3390/ijms23063058. Komsa-Penkova, R.; Stavreva, G.; Belemezova, K.; Kyurkchiev, S.; Todinova, S.; Altankov, G. Mesenchymal Stem-Cell Remodeling of Adsorbed Type-I Collagen—The Effect of Collagen Oxidation. International Journal of Molecular Sciences 2022, 23, 3058, doi:10.3390/ijms23063058.

Abstract

Abstract: The effect of collagen type 1 (Col I) oxidation on Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) remodeling is described as a model for acute oxidative stress. Morphologically, remodeling was presented by a mechanical rearrangement of adsorbed FITC-Col I and a trend for its organization in a fibril-like pattern - a process strongly abrogated in oxidized samples, but without visible changes in cell morphology. The cellular proteolytic activity was quantified in multiple samples utilizing fluorescence de-quenching (FRET effect). In the presence of ADMSCs a significant increase of native FITC-Col I fluorescence was observed, almost absent in the oxidized samples. Parallel studies in cell-free systems confirmed the enzymatic de-quenching of native FITC-Col I by Clostridial collagenase, again showing significant inhibition in oxidized samples. The structural changes in the oxidized Col I was further studied by Differential Scanning Calorimetry: an additional endotherm at 33.6°C along with the typical for native Col I at 40.5°C with sustained enthalpy (∆H) was observed in oxidized samples. Collectively, it has been evidenced that remodeling of Col I by ADMSCs is altered upon oxidation due to the intrinsic changes in the protein structure, thus presenting a novel mechanism for the control of stem cells' behavior toward collagen.

Keywords

Mesenchymal stem cells; collagen type I; remodeling; oxidation

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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