Komsa Penkova, R.; Stavreva, G.; Kyurkchiev, S.; Belemezova, K.; Todinova, S.; Altankov, G. Mesenchymal Stem Cells Remodeling of Adsorbed Type I Collagen – Effect of Collagen Oxidation. Preprints2021, 2021100276. https://doi.org/10.20944/preprints202110.0276.v1
Komsa Penkova, R., Stavreva, G., Kyurkchiev, S., Belemezova, K., Todinova, S., & Altankov, G. (2021). Mesenchymal Stem Cells Remodeling of Adsorbed Type I Collagen – Effect of Collagen Oxidation. Preprints. https://doi.org/10.20944/preprints202110.0276.v1
Komsa Penkova, R., Svetla Todinova and George Altankov. 2021 "Mesenchymal Stem Cells Remodeling of Adsorbed Type I Collagen – Effect of Collagen Oxidation" Preprints. https://doi.org/10.20944/preprints202110.0276.v1
Abstract: The effect of collagen type 1 (Col I) oxidation on Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) remodeling is described as a model for acute oxidative stress. Morphologically, remodeling was presented by a mechanical rearrangement of adsorbed FITC-Col I and a trend for its organization in a fibril-like pattern - a process strongly abrogated in oxidized samples, but without visible changes in cell morphology. The cellular proteolytic activity was quantified in multiple samples utilizing fluorescence de-quenching (FRET effect). In the presence of ADMSCs a significant increase of native FITC-Col I fluorescence was observed, almost absent in the oxidized samples. Parallel studies in cell-free systems confirmed the enzymatic de-quenching of native FITC-Col I by Clostridial collagenase, again showing significant inhibition in oxidized samples. The structural changes in the oxidized Col I was further studied by Differential Scanning Calorimetry: an additional endotherm at 33.6°C along with the typical for native Col I at 40.5°C with sustained enthalpy (∆H) was observed in oxidized samples. Collectively, it has been evidenced that remodeling of Col I by ADMSCs is altered upon oxidation due to the intrinsic changes in the protein structure, thus presenting a novel mechanism for the control of stem cells' behavior toward collagen.
Mesenchymal stem cells; collagen type I; remodeling; oxidation
Biology and Life Sciences, Biology and Biotechnology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.