Mesenchymal Stem Cells Remodeling of Adsorbed Type I Collagen – Effect of Collagen Oxidation

Regina Komsa Penkova; Galya Stavreva; Stanimir Kyurkchiev; Kalina Belemezova; Svetla Todinova; George Altankov

doi:10.20944/preprints202110.0276.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 16 October 2021 / Approved: 19 October 2021 / Online: 19 October 2021 (12:19:16 CEST)

Abstract: The effect of collagen type 1 (Col I) oxidation on Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) remodeling is described as a model for acute oxidative stress. Morphologically, remodeling was presented by a mechanical rearrangement of adsorbed FITC-Col I and a trend for its organization in a fibril-like pattern - a process strongly abrogated in oxidized samples, but without visible changes in cell morphology. The cellular proteolytic activity was quantified in multiple samples utilizing fluorescence de-quenching (FRET effect). In the presence of ADMSCs a significant increase of native FITC-Col I fluorescence was observed, almost absent in the oxidized samples. Parallel studies in cell-free systems confirmed the enzymatic de-quenching of native FITC-Col I by Clostridial collagenase, again showing significant inhibition in oxidized samples. The structural changes in the oxidized Col I was further studied by Differential Scanning Calorimetry: an additional endotherm at 33.6°C along with the typical for native Col I at 40.5°C with sustained enthalpy (∆H) was observed in oxidized samples. Collectively, it has been evidenced that remodeling of Col I by ADMSCs is altered upon oxidation due to the intrinsic changes in the protein structure, thus presenting a novel mechanism for the control of stem cells' behavior toward collagen.

Mesenchymal stem cells; collagen type I; remodeling; oxidation

LIFE SCIENCES, Biotechnology

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Mesenchymal Stem Cells Remodeling of Adsorbed Type I Collagen – Effect of Collagen Oxidation

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