Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars

Version 1 : Received: 12 October 2021 / Approved: 14 October 2021 / Online: 14 October 2021 (08:38:05 CEST)

How to cite: Beketova, M.P.; Chalaya, N.A.; Zoteyeva, N.M.; Gurina, A.A.; Kuznetsova, M.A.; Armstrong, M.; Hein, I.; Drobyazina, P.E.; Khavkin, E.E.; Rogozina, Е.V. Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars. Preprints 2021, 2021100209 (doi: 10.20944/preprints202110.0209.v1). Beketova, M.P.; Chalaya, N.A.; Zoteyeva, N.M.; Gurina, A.A.; Kuznetsova, M.A.; Armstrong, M.; Hein, I.; Drobyazina, P.E.; Khavkin, E.E.; Rogozina, Е.V. Combination Breeding and Marker-Assisted Selection to Develop Late Blight Resistant Potato Cultivars. Preprints 2021, 2021100209 (doi: 10.20944/preprints202110.0209.v1).

Abstract

(1) Background: Although resistance to pathogens and pests has been researched in many potato cultivars and breeding lines with DNA markers, there is scarce evidence as to the efficiency of the marker-assisted selection (MAS) for these traits when applied at the early stages of breeding. A goal of this study was to estimate the potential of affordable DNA markers to track Rpi disease resistance genes, that are effective against the pathogen Phytophthora infestans, as a practical breeding tool on a progeny of 68 clones derived from a cross between the cultivar Sudarynya and 13/11-09. (2) Methods: this population was studied for four years to elucidate the distribution of LB resistance and other agronomical desirable or simple to phenotype traits such as tuber and flower pigmentation, capacity and structure of yield. LB resistance was phenotypically determined through natural and artificial infection and the presence/absence of nine Rpi genes was assessed via 11 sequence-characterized amplified region (SCAR) markers. To aid this analysis, the profile of Rpi genes in the 13/11-09 parent was established using diagnostic resistance gene enrichment sequencing (dRenSeq) as a gold standard. (3) Results: at the early stages of a breeding program, MAS can halve the workload when screening the segregation of F1 offspring and selected SCAR markers for Rpi-genes provide useful tools.

Keywords

Rpi-genes; parental lines; hybrid progeny; dRenSeq; SCAR markers.

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