Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transcriptome-wide Identification and Quantification of Caffeoylquinic Acid Biosynthesis Pathway and Prediction of their Putative BAHDs Gene Complex in A. spathulifolius

Version 1 : Received: 21 May 2021 / Approved: 21 May 2021 / Online: 21 May 2021 (15:13:15 CEST)

A peer-reviewed article of this Preprint also exists.

Claude, S.-J.; Park, S.; Park, S.-J. Transcriptome-Wide Identification and Quantification of Caffeoylquinic Acid Biosynthesis Pathway and Prediction of Its Putative BAHDs Gene Complex in A. spathulifolius. Int. J. Mol. Sci. 2021, 22, 6333. Claude, S.-J.; Park, S.; Park, S.-J. Transcriptome-Wide Identification and Quantification of Caffeoylquinic Acid Biosynthesis Pathway and Prediction of Its Putative BAHDs Gene Complex in A. spathulifolius. Int. J. Mol. Sci. 2021, 22, 6333.

Journal reference: Int. J. Mol. Sci. 2021, 22, 6333
DOI: 10.3390/ijms22126333

Abstract

The phenylpropanoid pathway is a major secondary metabolite pathway that helps plants overcome biotic and abiotic stress and produces various by-products that promote human health. Its byproduct, chloroquinic acid (CQA), is a soluble phenolic compound present in many angiosperms. Hy-droxycinnamate-CoA shikimate/quinate transferase(BAHDs superfamily enzyme) is a significant en-zyme that plays a role in accumulating CQA biosynthesis. This study analyzed transcriptome-wide identification of the phenylpropanoid to chloroquinic acid biosynthesis candidate genes in A. spathulifolius flowers and leaves. Transcriptomic analyses of the flowers and leaves showed a differential expression of the PPP and CQA biosynthesis regulated unigenes. An analysis of PPP captive unigenes revealed the following: the major duplication of the key enzyme, PAL, 120 unigenes in leaves and 76 in flowers; the gene encoding C3’H, 169 unigenes in leaves and 140 unigenes in flowers; duplicated unigenes of 4CL, 41 in leaves and 27 in flowers. In addition, C4H unigenes had 12 unigenes in the leaves of A. spathulifolius and four in the flowers. The characterization of the BAHDs superfamily members identified 82 in leaves and 72 in flowers. Among them, phylogenetic analysis showed that five unigenes encoded HQT and three en-coded HCT in A. spathulifolius. The three HQT are common to both leaves and flowers, whereas the two HQT were specialized for leaves. The pattern of HQT synthesis was upregulated in flowers, whereas HCT was expressed strongly in the leaves of A. spathulifolius. Overall, 4CL, C4H, and HQT are expressed strongly in flowers, and caffeic acid and HCT show more expression in leaves. Therefore, CQA biosynthesis occurs in the flowers of A. spathulifolius rather than leaves.

Subject Areas

Phenylpropanoid pathway; Caffeoylquinic acid; BAHDs; hydroxycinnamoyl-coenzyme A: quinate hydroxycinnamoyl transferase; hydroxycinnamoyl-coenzyme A: shikimate/quinate hy droxycinnamoyl transferase;

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