Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Liver Proteome Profile of Growth Restricted and Appropriately Grown Newborn Wistar Rats Associated with Maternal Undernutrition

Polyxeni-Maria Sarli
* ,
Antigoni Manousopoulou
,
Elias Efthymiou
,
Andreas Zouridis
,
Anastasios Potiris
ORCID logo ,
Panagiota Pervanidou
,
Konstantinos Panoulis
,
Nikolaos Vlahos
,
Efthimios K. Deligeoroglou
,
Spiros D. Garbis
,
Makarios Eleftheriades
*
Version 1 : Received: 27 February 2021 / Approved: 1 March 2021 / Online: 1 March 2021 (13:54:56 CET)

How to cite: Sarli, P.-M.; Manousopoulou, A.; Efthymiou, E.; Zouridis, A.; Potiris, A.; Pervanidou, P.; Panoulis, K.; Vlahos, N.; Deligeoroglou, E. K.; Garbis, S. D.; Eleftheriades, M. Liver Proteome Profile of Growth Restricted and Appropriately Grown Newborn Wistar Rats Associated with Maternal Undernutrition. Preprints 2021, 2021030023. https://doi.org/10.20944/preprints202103.0023.v1 Sarli, P.-M.; Manousopoulou, A.; Efthymiou, E.; Zouridis, A.; Potiris, A.; Pervanidou, P.; Panoulis, K.; Vlahos, N.; Deligeoroglou, E. K.; Garbis, S. D.; Eleftheriades, M. Liver Proteome Profile of Growth Restricted and Appropriately Grown Newborn Wistar Rats Associated with Maternal Undernutrition. Preprints 2021, 2021030023. https://doi.org/10.20944/preprints202103.0023.v1

Abstract

1) Background: Fetal Growth Restriction (FGR) has been associated with adverse perinatal outcomes and epigenetic modifications that impact gene expression leading to permanent changes of fetal metabolic pathways and thereby influence development of disease in childhood and adult life. Both clinical and experimental studies showed that maternal nutrition during pregnancy is critical since malnutrition adversely affects fetal growth and physiology. In this study, we investigated the result of maternal food restriction on liver protein expression in Wistar male newborn pups. (2) Materials & methods: Pups born to food restricted mothers were subdivided to FGR and non-FGR groups. Livers of control, FGR and non-FGR groups were analyzed using quantitative proteomics. (3) Results: In total 6665 proteins were profiled. Of these, 451 and 751 were differentially expressed in FGR and non-FGR vs. control respectively, whereas 229 were common between the two groups. Bioinformatics analysis of the differentially expressed proteins (DEPs) in FGR vs. control revealed: induction of the super-pathway of cholesterol biosynthesis and inhibition of thyroid hormone metabolism, fatty acid beta oxidation and apelin liver signaling pathway. In the DEPs of non-FGR vs. control groups there was inhibition of thyroid hormone metabolism, fatty acid beta oxidation and apelin liver signaling pathway as well. (4) Conclusion: This study demonstrates the impact of prenatal food restriction on the proteomic liver profile of FGR and non-FGR offspring underlying the importance of both prenatal adversities and birth weight on liver dependent postnatal disease.

Keywords

FGR; fetal programming; food restriction; metabolic disorders; liver proteomics

Subject

Medicine and Pharmacology, Immunology and Allergy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.