Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Salinity and Short-Term Exposure to High Temperature Altered Dead Pericarp and Diminished Yield of the Crop Plant Brassica Juncea

Version 1 : Received: 21 February 2021 / Approved: 22 February 2021 / Online: 22 February 2021 (11:48:15 CET)

How to cite: Swetha, B.; Singiri, J.R.; Novoplansky, N.; Grandhi, R.; Srinivasan, J.; Khadka, J.; Sikron-Persi, N.; Galis, I.; Grafi, G. Salinity and Short-Term Exposure to High Temperature Altered Dead Pericarp and Diminished Yield of the Crop Plant Brassica Juncea. Preprints 2021, 2021020461 (doi: 10.20944/preprints202102.0461.v1). Swetha, B.; Singiri, J.R.; Novoplansky, N.; Grandhi, R.; Srinivasan, J.; Khadka, J.; Sikron-Persi, N.; Galis, I.; Grafi, G. Salinity and Short-Term Exposure to High Temperature Altered Dead Pericarp and Diminished Yield of the Crop Plant Brassica Juncea. Preprints 2021, 2021020461 (doi: 10.20944/preprints202102.0461.v1).

Abstract

Climate change is expected to increase the frequency and severity of abiotic stresses that lead to loss of crop yield. We investigated the effect of salinity (S), short episodes of high temperature (HS) and combination of S+HS at the reproductive phase on dead pericarps properties and yield of the crop plant Brassica juncea. Three intervals of HS resulted in massive seed abortion; seeds from salt-treated plants germinated poorly. Pericarp extracts of salt-treated plants reduced seed germination of B. juncea; all pericarp extracts completely inhibited seed germination of tomato and Arabidopsis; removal of pericarp extracts restored seed germination. HS reduced all metabolites accumulated in dead pericarps, except for upregulation of isomaltose and cellobiose. Salt induced alteration in metabolite levels including increase in proline, reduction in TCA intermediates and changes in phytohormone levels. Proteome analysis revealed hundreds of proteins stored in dead pericarps whose levels and composition were altered under salt stress. The integration of metabolic and proteomic data showed that changes in metabolites were highly correlated with changes in proteins involved in their biosynthetic pathways. Thus, besides providing a physical shield for seed/embryo protection dead pericarps store beneficial substances whose levels, composition and biological function are altered under stress, further highlighting the elaborated function of dead organs enclosing embryos in seed biology and ecology. The detrimental effect of HS on crop production might have implications for global food security in the face of climate change.

Subject Areas

Dead pericarps; salinity; short episodes of high temperature; stress response; reproductive phase; seed abortion; Phyohormones; Climate change; Brassica juncea

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.