Article
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Detection of Chymotrypsin by Optical and Acoustic Methods
Version 1
: Received: 30 January 2021 / Approved: 1 February 2021 / Online: 1 February 2021 (13:59:40 CET)
A peer-reviewed article of this Preprint also exists.
Piovarci, I.; Hianik, T.; Ivanov, I.N. Detection of Chymotrypsin by Optical and Acoustic Methods. Biosensors 2021, 11, 63. Piovarci, I.; Hianik, T.; Ivanov, I.N. Detection of Chymotrypsin by Optical and Acoustic Methods. Biosensors 2021, 11, 63.
Abstract
Chymotrypsin is an important proteolytic enzyme in human digestion system that cleaves milk proteins, through hydrolysis reaction, which makes it interesting subject to study activity of milk proteases. In this work, we compared detection of chymotrypsin by spectrophotometric, dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods and determined the limit of chymotrypsin detection (LOD), 0.15 ± 0.01 nM for spectrophotometric, 0.67 ± 0.05 nM for DLS and 1.40 ± 0.30 nM for QCM methods, respectively. We discuss peculiarities and give perspective for implementation of detection methods and note that while the optical detection methods are simple to implement, the QCM method is more robust for sample preparation. We give an overview on methods and instruments for detection of chymotrypsin and other milk proteases.
Keywords
chymotrypsin; β-casein; nanoparticles; UV-Vis spectroscopy; dynamic light scattering; quartz crystal microbalance.
Subject
Biology and Life Sciences, Biophysics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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