Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Detection of Chymotrypsin by Optical and Acoustic Methods

Version 1 : Received: 30 January 2021 / Approved: 1 February 2021 / Online: 1 February 2021 (13:59:40 CET)

A peer-reviewed article of this Preprint also exists.

Piovarci, I.; Hianik, T.; Ivanov, I.N. Detection of Chymotrypsin by Optical and Acoustic Methods. Biosensors 2021, 11, 63. Piovarci, I.; Hianik, T.; Ivanov, I.N. Detection of Chymotrypsin by Optical and Acoustic Methods. Biosensors 2021, 11, 63.

Journal reference: Biosensors 2021, 11, 63
DOI: 10.3390/bios11030063

Abstract

Chymotrypsin is an important proteolytic enzyme in human digestion system that cleaves milk proteins, through hydrolysis reaction, which makes it interesting subject to study activity of milk proteases. In this work, we compared detection of chymotrypsin by spectrophotometric, dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods and determined the limit of chymotrypsin detection (LOD), 0.15 ± 0.01 nM for spectrophotometric, 0.67 ± 0.05 nM for DLS and 1.40 ± 0.30 nM for QCM methods, respectively. We discuss peculiarities and give perspective for implementation of detection methods and note that while the optical detection methods are simple to implement, the QCM method is more robust for sample preparation. We give an overview on methods and instruments for detection of chymotrypsin and other milk proteases.

Subject Areas

chymotrypsin; β-casein; nanoparticles; UV-Vis spectroscopy; dynamic light scattering; quartz crystal microbalance.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.