Version 1
: Received: 25 January 2021 / Approved: 26 January 2021 / Online: 26 January 2021 (10:01:35 CET)
How to cite:
Piccirella, S.; Cristoni, S.; Fowler, C.; Doecke, J.; Abate, G.; Uberti, D. Discovery of Simple Blood-Based Test to Predict Early Onset of Alzheimer’s Using Standard Clinical Mass Spectrometry Platforms. Preprints2021, 2021010530 (doi: 10.20944/preprints202101.0530.v1).
Piccirella, S.; Cristoni, S.; Fowler, C.; Doecke, J.; Abate, G.; Uberti, D. Discovery of Simple Blood-Based Test to Predict Early Onset of Alzheimer’s Using Standard Clinical Mass Spectrometry Platforms. Preprints 2021, 2021010530 (doi: 10.20944/preprints202101.0530.v1).
Cite as:
Piccirella, S.; Cristoni, S.; Fowler, C.; Doecke, J.; Abate, G.; Uberti, D. Discovery of Simple Blood-Based Test to Predict Early Onset of Alzheimer’s Using Standard Clinical Mass Spectrometry Platforms. Preprints2021, 2021010530 (doi: 10.20944/preprints202101.0530.v1).
Piccirella, S.; Cristoni, S.; Fowler, C.; Doecke, J.; Abate, G.; Uberti, D. Discovery of Simple Blood-Based Test to Predict Early Onset of Alzheimer’s Using Standard Clinical Mass Spectrometry Platforms. Preprints 2021, 2021010530 (doi: 10.20944/preprints202101.0530.v1).
Abstract
Despite the increasing number of individuals affected by Alzheimer’s disease (AD) every year, no effective therapy has been developed to treat this neurodegenerative disease yet. The current methods for AD diagnosis are effective for clinical confirmation of the disease only when symptoms become apparent, years after molecular damage started within the patients’ brains. As higher expression of a conformationally altered p53 has been correlated with AD, we developed a mass spectrometry-based method for highly sensitive, specific, and reproducible quantification of a p53 conformational variant in plasma samples of patients with known clinical outcome. In particular, we tested the prognostic performance of an AD-specific 2D3A8-immunoselected p53 peptide (AZ 284™) in different sets of individuals progressing from both cognitively unimpaired (CU) and mild cognitive impairment (MCI) patients progressing to AD dementia. Our data showed that quantitative analysis of AZ 284™ is a reliable tool for predicting AD progression up to 6 years prior to dementia onset with AUC >90%. Taken together, these results support the implementation of p53 conformational variant quantification as an affordable and powerful diagnostic tool for early, non-invasive AD diagnosis.
Keywords
Alzheimer’s disease; biomarker; prognosis; P53; mass spectrometry
Subject
MEDICINE & PHARMACOLOGY, Clinical Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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